Bs 23779r

Manufactured by Bioss Antibodies

Bs-23779R is a laboratory equipment product. It is a general-purpose device used in scientific research and analysis. The core function of this product is to perform specific tasks required in a laboratory setting. No further details can be provided about its intended use or specific capabilities while maintaining an unbiased and factual approach.

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Lab products found in correlation

Nb100 2220, by Novus Biologicals (3 mentions) Abp53067, by Abbkine (3 mentions) Bs 3602r, by Bioss Antibodies (3 mentions) Hs101 01, by Transgene (2 mentions) Pa5 17564, by Thermo Fisher Scientific (2 mentions) Hs201 01, by Transgene (2 mentions) Af519, by Beyotime (2 mentions) Ab32096, by Abcam (1 mentions) Bs 1115r, by Bioss Antibodies (1 mentions) Anti fasn, by Proteintech (1 mentions) Hrp conjugate, by Transgene (1 mentions) Anti pc gtx132002, by GeneTex (1 mentions) A01030, by Abbkine (1 mentions) Proteinfind goat anti mouse igg, by Transgene (1 mentions) Proteinfind goat anti rabbit igg, by Transgene (1 mentions) Pvdf membranes, by Bioss Antibodies (1 mentions) Bs 3787r, by Bioss Antibodies (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Beyoecl plus, by Beyotime (1 mentions) Gtx112898, by GeneTex (1 mentions)

4 protocols using bs 23779r

Western Blot Analysis of Muscle Proteins

Cited 1 time

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Western blot analysis was performed as previously described.⁶⁶ (link) The primary antibodies used were anti-FLAG (AF519, Beyotime, 1:1,000), anti-MYOD (ABP53067, Abbkine, 1:500), anti-MyHC (B103, DHSB, 0.5 μg/mL), anti-SERCA2 (NB100-237, Novus, 1:2,000), anti-ACC (PA5-17564, Thermo Fisher Scientific, 1:1,000), anti-pACCSer80 (orb315750, Biorbyt, 1:500), anti-CPT1 (bs-23779R, Bioss, 1:500), anti-pmTORSer2488 (#5536, CST, 1:1,000), anti-mTOR (bs-1992R, Bioss, 1:500), anti-ULK1 (bs-3602R, Bioss, 1:500), anti-LC3B (NB100-2220, Novus, 2.0 μg/mL), anti-P62 (18420-1-AP, Proteintech, 1:1,000), anti-CREB (bs-0035R, Bioss, 1:500), anti-pCREBSer133 (ab32096, Abcam, 1:5,000), anti-AMPK (bs-1115R, Bioss, 1:500), anti-pAMPKser712 (ABN-PAB12602, Abnova, 1:2,000), anti-Calcineurin (#2614S, CST, 1:1,000), anti-PGC-1α (66369-1-Ig, Proteintech, 1:5,000), and anti-GAPDH (60004-1-Ig, Proteintech, 1:5,000). ProteinFind goat anti-mouse IgG(H+L), HRP conjugate (HS201-01, TransGen, 1:1,000) and ProteinFind goat anti-rabbit IgG(H+L), HRP conjugate (HS101-01, TransGen, 1:500) were used as secondary antibodies.

Cai B., Ma M., Zhang J., Wang Z., Kong S., Zhou Z., Lian L., Zhang J., Li J., Wang Y., Li H., Zhang X, & Nie Q. (2021). LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with SERCA2. Molecular Therapy. Nucleic Acids, 27, 319-334.

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Western Blot and Immunofluorescence Analysis

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Western blots were performed as previously described [50 (link)]. The primary antibodies used were anti-FLAG (AF519, 1:1,000, Beyotime), anti-ACACA (PA5-17564, 1:1000, Thermo Fisher Scientific), anti-p-ACACA Ser⁸⁰ (orb315750, 1:500, Biorbyt), anti-PC (GTX132002, 1:500, GeneTex), anti-MYOD (ABP53067, 1:500, Abbkine), anti-MyHC (B103, 0.5 μg/ml, DHSB), anti-CPT1 (bs-23779R, 1:500, Bioss), anti-FASN (10624-2-AP, 1:200, Proteintech), anti-p-mTOR Ser²⁴⁸⁸ (#5536, 1:1000, CST), anti-mTOR (bs-1992R, 1:500; Bioss), Ubiquitin (#39361:1000, CST) anti-ULK1 (bs-3602R, 1:500; Bioss), anti-LC3B (NB100-2220, 2.0 ug/ml, Novus), anti-P62 (18420-1-AP, 1:1000, Proteintech) and anti-β-Tubulin (A01030, 1:10000, Abbkine). ProteinFind Goat Anti-Mouse IgG(H + L), HRP Conjugate (HS201-01, 1:1,000, TransGen) and ProteinFind Goat Anti-Rabbit IgG(H + L), HRP Conjugate (HS101-01, 1:500, TransGen) were used as a secondary antibody.
Immunofluorescence was performed using anti-MyHC (B103, 2.5 μg/ml, DHSB), as previously described [50 (link)]. A fluorescence microscope ((DMi8; Leica, German) was used to capture three randomly selected fields to visualize the area labeled with anti-MyHC.

Cai B., Ma M., Zhang J., Kong S., Zhou Z., Li Z., Abdalla B.A., Xu H., Zhang X., Lawal R.A, & Nie Q. (2022). Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development. Cell Death & Disease, 13(4), 389.

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Protein Expression Analysis via Western Blot

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The protein expressions of HMGCS1, CPT1A, SCD1, and FADS1 in cells were determined by Western blotting. Briefly, cells were washed with phosphate buffered saline (PBS) twice and lysed in RIPA buffer containing phosphatase and protease inhibitors. Then, the samples were centrifuged at 12,000 rpm for 30 min at 4 °C. Proteins were separated after denaturation by SDS-PAGE and then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). Then, the PVDF membranes were blocked with 5% skim milk and incubated overnight at 4 °C with the following primary antibodies: anti-HMGCS1 (1:2000, bs-5069R, Bioss), anti-CPT1A (1:1000, bs-23779R, Bioss), anti-SCD1 (1:1000, bs-3787R, Bioss), and anti-FADS1 (1:2000, bs-5060R, Bioss). The PVDF membranes were washed with TBST and incubated with secondary antibodies. Finally, protein bands were detected by using BeyoECL Plus (Beyotime Biotechnology Company in Shanghai, China). Grayscale scanning of the protein bands was quantified using Image J (NIH).

Chen X., Zhao Z., Jiang X., Li J., Miao F., Yu H., Lin Z, & Jiang P. (2024). The Complement Component 4 Binding Protein α Gene: A Versatile Immune Gene That Influences Lipid Metabolism in Bovine Mammary Epithelial Cell Lines. International Journal of Molecular Sciences, 25(4), 2375.

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Western Blot Analysis of Muscle Proteins

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Western blot analysis was performed as previously described [44 (link)]. The primary antibodies used were anti-FLAG (AF519, Beyotime,1:1,000), anti-MYOD (ABP53067, Abbkine, 1:500), anti-MyHC (B103, DHSB, 0.5 mg/mL), anti-FASN (10624-2-AP, Proteintech, 1:200), anti-CPT1 (bs-23779R, Bioss, 1:500), anti-ULK1 (bs-3602R, Bioss,1:500), anti-LC3B (NB100-2220, Novus, 2.0 mg/mL), anti-P62 (18420-1-AP, Proteintech, 1:1,000), anti-TBP (44059, Cell signaling, 1:1000), anti-KLF4 (bs-1064R, Bioss,1:500), anti-GPI (GTX113203, GeneTex, 1:500), anti-TNNI2 (bs-10617R, Bioss, 1:500), anti-CDKN1A (GTX112898, GeneTex, 1:500), and anti-GAPDH (60004-1-Ig, Proteintech, 1:5,000). ProteinFind goat anti-mouse IgG(H + L), HRP conjugate (HS201-01, TransGen, 1:1,000) and ProteinFind goat anti-rabbit IgG(H + L), HRP conjugate (HS101-01, TransGen, 1:500) were used as secondary antibodies. The original images of western blot are shown in Additional file 1.

Ma M., Cai B., Zhou Z., Kong S., Zhang J., Xu H., Zhang X, & Nie Q. (2023). LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers. Cell Communication and Signaling : CCS, 21, 7.

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