A total of 13 human downy hairs were collected from one arm of a healthy donor (Asian male) [Fig. 1(a1)]. Each hair was gently posed on the top of a coverslip (24 × 50 mm 2 , C024501, Matsunami, LTD, Japan) with oil immersion (n ¼ 1.518), which decreases the RI contrast between the hairs and the medium. The sample was then covered with another coverslip [Fig. 1(a2)]. To study the effects of hydrogen peroxide on the hair structures, four hair samples were treated with 3% H 2 O 2 solution (Sigma-Aldrich, St. Louis, Missouri) for 24 h. The shape of the edges of the hairs cells were imaged before and after the H 2 O 2 treatments.
C024501
Manufactured by Matsunami
Sourced in Japan
The C024501 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, allowing for efficient separation of samples in various scientific and research settings.
Automatically generated - may contain errors
3 protocols using c024501
1
Morphological Effects of Hydrogen Peroxide on Downy Hairs
2
Fluorescent Sperm Motility Tracking
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Sperm movement trajectories were recorded according to our previously reported method with minor modifications [21 (link)]. Briefly, after nuclear staining
with SYBR14 (the final concentration: 120 nM, L7011, Thermo Fisher Scientific Inc., Waltham, MA, USA), a drop of sperm suspension (100 μl) was put on the pre-warmed silicon-coated glass
slide (76 × 26 mm, S6111, Matsunami Glass Ind., Ltd., Kishiwada, Japan) and covered with the coverslip (50 × 24 mm, C024501, Matsunami) gently to make sufficient space for sperm 3D
hyperactivation. The preparations of swimming spermatozoa were observed on a heated stage (38.5°C) of an upright microscope equipped with epifluorescence (U-FBW mirror unit composed of
BP460-495 excitation filter, DM505 dichroic mirror, and BA510IF emission filter, Olympus), and sperm movement trajectories were captured using a CCD camera (DP73, Olympus) with the exposure
time set to 2 sec.
with SYBR14 (the final concentration: 120 nM, L7011, Thermo Fisher Scientific Inc., Waltham, MA, USA), a drop of sperm suspension (100 μl) was put on the pre-warmed silicon-coated glass
slide (76 × 26 mm, S6111, Matsunami Glass Ind., Ltd., Kishiwada, Japan) and covered with the coverslip (50 × 24 mm, C024501, Matsunami) gently to make sufficient space for sperm 3D
hyperactivation. The preparations of swimming spermatozoa were observed on a heated stage (38.5°C) of an upright microscope equipped with epifluorescence (U-FBW mirror unit composed of
BP460-495 excitation filter, DM505 dichroic mirror, and BA510IF emission filter, Olympus), and sperm movement trajectories were captured using a CCD camera (DP73, Olympus) with the exposure
time set to 2 sec.
3
Imaging Pollen Grains using ODT
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The pollen grains were collected during late April and May from blooming pine trees of a local farm. Pollen grains were placed in containers by gently shaking them off the male cones of the trees. Due to the contemporarily humid climate, the pollen grains were dried for approximately 2 hours at room temperature to prevent microbial invasion to the pollen grains. The containers with pollen grains were sealed and preserved in a dark cabinet at room temperature before imaging. In order to prepare pollen grains for imaging, they were suspended in a drop of RI-matching oil (Series A, Cargille Labs, United States) and sandwiched between two cover glasses (C024501, Matsunami Glass Ind. Ltd., Japan). The use of oil enables index matching between the sample and surrounding media, providing the appropriate imaging condition for ODT by significantly reducing light scattering57 (link). The condition for drying and suspending pollen grains in oil does not significantly alter the physiological conditions58 (link)–60 . Imaging was conducted using pollen grains that were not aggregated with other particles.
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