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α tubulin 200 301 880

Manufactured by Rockland Immunochemicals
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α-tubulin (200-301-880) is a laboratory reagent produced by Rockland Immunochemicals. It is a protein component of the cytoskeleton in eukaryotic cells.

Automatically generated - may contain errors

Lab products found in correlation

Ab5095, by Abcam (2 mentions) Ab5131, by Abcam (2 mentions) Ab78272, by Abcam (2 mentions) Actin sc 1615, by Santa Cruz Biotechnology (2 mentions) Sc 9001, by Santa Cruz Biotechnology (2 mentions) Sc 28678, by Santa Cruz Biotechnology (2 mentions) Sc 484, by Santa Cruz Biotechnology (2 mentions)

2 protocols using α tubulin 200 301 880

1

Western Blot Analysis of Transcription

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously described 46 using approximately 30 μg of proteins from cells boiled in Laemmli buffer (50mM Tris pH6.8, 2% sodium dodecyl sulphate, 5% β-mercaptoethanol, 10% glycerol, 0.1% Bromophenol Blue) and antibodies against pol II (sc-9001, Santa Cruz), pSer2 (Ab5095, Abcam), pSer5 (ab5131, Abcam), mitotic phosphoproteins/CC3 (ab78272, Abcam), which recognizes Spt5P 44 (link), Spt5 (sc-28678, Santa Cruz), α-tubulin (200-301-880, Rockland), actin (sc-1615, Santa Cruz), Pcf11 (A303-706A, Bethyl Laboratories), Tyr1P (Active Motif 61384), Thr4P (Active motif 61362), Ser7P (Active motif 61088), Cstf64 (A301-092A, Bethyl Laboratories) and CDK9 (sc-484, Santa Cruz). Validation for all antibodies is provided on the manufacturers’ websites.
Laitem C., Zaborowska J., Isa N.F., Kufs J., Dienstbier M, & Murphy S. (2015). CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nature structural & molecular biology, 22(5), 396-403.
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2

Western Blot Analysis of Transcription

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously described 46 using approximately 30 μg of proteins from cells boiled in Laemmli buffer (50mM Tris pH6.8, 2% sodium dodecyl sulphate, 5% β-mercaptoethanol, 10% glycerol, 0.1% Bromophenol Blue) and antibodies against pol II (sc-9001, Santa Cruz), pSer2 (Ab5095, Abcam), pSer5 (ab5131, Abcam), mitotic phosphoproteins/CC3 (ab78272, Abcam), which recognizes Spt5P 44 (link), Spt5 (sc-28678, Santa Cruz), α-tubulin (200-301-880, Rockland), actin (sc-1615, Santa Cruz), Pcf11 (A303-706A, Bethyl Laboratories), Tyr1P (Active Motif 61384), Thr4P (Active motif 61362), Ser7P (Active motif 61088), Cstf64 (A301-092A, Bethyl Laboratories) and CDK9 (sc-484, Santa Cruz). Validation for all antibodies is provided on the manufacturers’ websites.
Laitem C., Zaborowska J., Isa N.F., Kufs J., Dienstbier M, & Murphy S. (2015). CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nature structural & molecular biology, 22(5), 396-403.
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