Tnfα 300 01a and 315 01a

Western blotting and immunoprecipitations were performed using the following primary homemade antibody: a monoclonal antibody against Morgana (P1PP0) was generated in our laboratory using GST-morgana fusion protein as an antigen^{20 (link)}. Other commercially antibodies were used: Vinculin (Sigma, SAB4200080, 1:5000), CHORDC1 (Sigma, HPA041040, 1:1000), MMP9 (Abcam, ab76003, 1:1000), MMP2 (Santa Cruz, 8835, 1:500), HSP90 (Santa Cruz, 13119, 1:1000), P-IkBα Ser32-36 (Santa Cruz, 8404, 1:500), P-IkBα Tyr42 (Abcam, ab24783, 1:1000), IkBα (Cell Signaling, 4814, 1:1000), IKKα (Cell Signaling, 11930, 1:1000), IKKα (Cell Signaling, 2682, 1:100 for co-immunoprecipitation experiments), IKKβ (Cell Signaling, 8943, 1:1000), IKKγ (Santa Cruz, 8032, 1:500), αTubulin (Sigma, T5168, 1:8000), MBP (Cell Signaling, 2396, 1:1000), TNF-R1 (Santa Cruz, 8436, 1:500), TAK-1 (Santa Cruz, 166562, 1:500), p-AKT (Cell Signaling, 9271, 1:1000), AKT (Cell Signaling, 4691, 1:1000), CDC37 (Santa Cruz, 5617, 1:500), P-IKKα/β (Cell Signaling, 2697, 1:1000).
TNFα (300-01A and 315-01A) was purchased from Peprotech, the IKKβ inhibitor PS1145 (P6624), ROCK inhibitor Y27632 (Y0503), the PTEN inhibitor VO-OHpic (V8639), and the AKT inhibitor GSK69093 (SML0428) were from Sigma. BEZ235 (S1009) was purchased from Selleckchem.

Commercially antibodies were used: Vinculin (Sigma, SAB4200080, 1:8000), IκBα (Cell Signaling, #9242, 1:1000), P65 (Cell Signaling, #8242, 1:1000), FLAG (abcam, ab1162), 1:1000), Myc (Cell Signaling, #2276, 1:1000), MITOFUSIN1 (Cell Signaling, #14739, 1:1000), OPA1 (Cell Signaling, #67589, 1:1000), Total OXPHOS Rodent WB Antibody Cocktail antibodies (Abcam, ab110413, 1:1000), peroxidase-conjugated secondary mouse (Cell Signaling, #7076, 1:2000) and peroxidase-conjugated secondary Rabbit (Cell Signaling, #7074, 1:2000). TNFα (300-01A and 315-01A) was purchased from Peprotech. Cisplatin (S1166) was purchased from Selleckchem. ZINC639309 (MolPort-002-836-946) was purchased from MolPort and Psammaplin-A (SC-258049) was purchased from Santa Cruz. NAC (N-acetylcysteine) was purchased from Sigma Aldrich.