Anti ha polyclonal antibody

LY294002 (MERCK Millipore; Damstadt, Germany), NSC23766, BKM120, BYL719, and MK2206 (Selleckchem; Houston, TX, USA) were used as PI3 kinase signaling inhibitors. MG132 (A.G. Scientific; San Diego, CA, USA) was used as a proteasome inhibitor. Recombinant human BMP2 was obtained from Cellumed (Seoul, Korea) and Insulin–transferrin–selenium (ITS) supplements for chondrocyte maturation were purchased from Thermo Fisher (Waltham, MA, USA). Anti-HA polyclonal antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-V5 monoclonal- and anti-Myc polyclonal antibodies were obtained from Merck Millipore (Damstadt, Germany). Anti-Flag monoclonal antibody was purchased from Sigma (St. Louis, MO, USA). Anti-Type X collagen (COL 10) antibody (Cosmobio; Tokyo, Japan) was used for immunohistochemistry (IHC). Anti-GAPDH and anti-β-actin antibodies (AbFrontier; Seoul, Korea) were used as loading controls for Western blotting. Anti-Nkx3.2 antibody purchased from Abcam (Cambridge, UK) and custom-made anti-Nkx3.2 antibody was obtained from Cosmogenetech (Seoul, Korea). Horseradish peroxidase (HRP)-conjugated anti-mouse IgG and anti-rabbit IgG were purchased from Cell Signaling (Danvers, MA, USA) and Cy3-conjugated anti-rabbit IgG was purchased from Jackson ImmunoResearch Inc. (West Grove, PA, USA).