Gc 2014 plus

The PHA content and monomers composition were analyzed using the methanolysis technique following Braunegg et al.^{62 (link)}. Briefly, 15–20 mg of lyophilized cells were added to the test tube, followed by 2 mL of chloroform and methanolysis solution (mixture of 85% v/v of methanol and 15% w/v H₂SO₄). The tubes were heated at 100 ° C for 180 min, then cooled at room temperature. After that, 1 mL of DW was added into the tubes and mixed vigorously for 1 min using a vortex mixer. The chloroform-rich PHA in the bottom layer was collected using a pasture pipette. Then, remove the water residues using Na₂SO₄. The mixture solution of 500 mL of chloroform-rich PHA solution and 500 mL of 0.2% (v/v) caprylic methyl ester (CME) (Internal standard) was prepared for GC analysis. The analysis was performed using Shimadzu GC-2014 plus (Shimadzu, Japan) supplied with Restek RTX-1 column (Restek, USA) and flame ionization detector (FID). The 2.0 µL of the prepared sample solution was injected into the GC machine. Nitrogen was used as a carrier gas for GC analysis. Besides, the injector and detector temperatures were set at 270 °C and 280 °C, respectively.