Mass spectrometry was performed using a Voyager-DE Pro MALDI-TOF mass spectrometer (AB Sciex) equipped with a nitrogen UV laser (λ=337 nm, 3 ns pulse). The instrument was operated in the positive-linear mode (mass accuracy: 0.05%) with an accelerating potential of 20 kV. Typically, mass spectra were obtained by accumulation of 600 laser shots for each analysis and processed using Data Explorer 4.0 software (AB Sciex). After desalting with C4 ZipTips (Millipore), samples were mixed with sinapinic acid (saturated solution in 30% acetonitrile and 0.3% trifluoroacetic acid), deposited on the MALDI target and air-dried before analysis. Errors are based on precision as stated by the manufacturer, as well as statistical errors, as a function of signal-to-noise, based on multiple repeats.
Voyager de pro maldi tof mass spectrometer
Manufactured by AB Sciex
The Voyager-DE Pro MALDI-TOF mass spectrometer is a tool used for the analysis of biomolecules. It utilizes matrix-assisted laser desorption/ionization (MALDI) and time-of-flight (TOF) mass spectrometry techniques to provide accurate mass measurements of various compounds, including proteins, peptides, and oligonucleotides.
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2 protocols using voyager de pro maldi tof mass spectrometer
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MALDI-TOF Mass Spectrometry Characterization
2
MALDI-TOF Analysis of Biomolecular Samples
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A Voyager-DE PRO MALDI TOF mass
spectrometer (AB Sciex, Framingham, MA) was utilized for LDI data.
Filtered/lyophilized day 0, 1, and 3 samples were resuspended in nanopure
water at a concentration of 0.2 mg mL–1 and tested
without any matrix. To this end, 10 μL of sample solution was
dropped onto a MALDI plate and dried under ambient conditions. For
data acquisition, the instrument settings were positive reflector
mode; 25 000 V accelerating voltage, grid voltage equals 75%
accelerating voltage, 1.12 mirror to accelerating voltage ratio, 200
ns extraction delay, and 2500 laser intensity with N2 laser
source.
spectrometer (AB Sciex, Framingham, MA) was utilized for LDI data.
Filtered/lyophilized day 0, 1, and 3 samples were resuspended in nanopure
water at a concentration of 0.2 mg mL–1 and tested
without any matrix. To this end, 10 μL of sample solution was
dropped onto a MALDI plate and dried under ambient conditions. For
data acquisition, the instrument settings were positive reflector
mode; 25 000 V accelerating voltage, grid voltage equals 75%
accelerating voltage, 1.12 mirror to accelerating voltage ratio, 200
ns extraction delay, and 2500 laser intensity with N2 laser
source.
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