After recovery from surgical trauma and acclimatization, rats in groups IV and V were connected to the recording cables and bipolar EEG, EOG, and EMG recordings were made between 10:00 A.M. and 10:00 P.M. using a Grass Model 7 polygraph following published methods (Mallick et al., 2001 (link); Pal and Mallick, 2009 (link)). The recordings were performed under control (without any treatment) and experimental (after treatment as described below) conditions. The electrophysiological signals were also recorded digitally at the sampling rate of 128 Hz using Vital Recording Software and later analyzed manually by SleepSign Software (Kissei Comtec).
Grass model 7 polygraph
Manufactured by Natus
Sourced in United States
The Grass Model 7 polygraph is a lab equipment product designed to record and measure physiological data. It is capable of simultaneously recording multiple channels of information, such as heart rate, respiration, and skin conductance. The device is intended for use in research and clinical settings.
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Lab products found in correlation
9 protocols using grass model 7 polygraph
1
Electrophysiological Recordings in Rats
2
Tracheal Contractility Assay for Allergic Responses
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Tracheal contractions were carried out as previously described3 . For allergen (ovalbumin, OVA) responses, mice were actively sensitized by injecting 0.2 mL of an OVA solution (3.75 μg/mL) mixed with Al(OH)3 three times at an interval of 2 days. Experiments were conducted on male and female animals 8-12 weeks of age beginning two weeks following the first injection. Trachea were cleaned of connective tissue and tracheal rings (whole or laterally divided in half), were suspended between two tungsten stirrups in 10 mL organ chambers filled with Krebs’ that was warmed to 37°C and bubbled with 95% O2-5% CO2 to maintain a pH of 7.4. One stirrup was connected to a strain gauge (model FT03; Grass Instruments, Quincy, MA), and tension was recorded on a Grass Model 7 polygraph (Grass Instruments, Quincy, MA). Preparations were stretched to a resting tension of 0.2 g, and washed with fresh Krebs’ buffer at 15-minute intervals during a 60 minute equilibration period. After equilibration, trachea were challenged with either OVA (10 μg/mL), or Compound 48/80. At the end of each experiment, all trachea were maximally contracted with carbachol (1 μM). All results are expressed as a percentage of maximum contraction.
3
Tracheal Contractility Assessment Protocol
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Soon after dissection of trachea, approximately 2–3 mm wide tracheal tube rings were cut down, each ring containing two cartilages were opened further by longitudinal incision to prepare tracheal strips [36 (link)]. Single tracheal strip was suspended in tissue bath (20 mL) containing standard physiological kreb’s solution, continuously supplied with carbogen and maintained at 37 °C (pH 7.4). Kreb’s solution used for this experiment was composed of (mM) glucose: 11.7, KH2PO4: 1.2, NaCl: 118.2, CaCl2: 2.5, MgSO4. 7H2O: 1.2, NaHCO3: 25.0, KCl: 4.7. Strips were permitted to equilibrate for almost 1 h before applying any test substance. Throughout experiment tracheal strips were applied with tension of 1 g. After the application of spasmogens like CCh and/or high K+, when sustained contractions were achieved then spasmolytic effect of drug was tested by its addition in cumulative manner. Grass model 7 Polygraph (USA) was used for recording the isometric responses of tracheal strips.
4
Isolated Atrium Contractility Assay
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Right atrium was isolated and suspended through wire gauze in 20 mL tissue bath containing Krebs solute, maintained at 32 °C continuously supplied with carbogen gas. Due to presence of natural pacemaker in the atrium, Spontaneous beating was observed under the resting tension of 1 g [38 (link)]. After 45 min of equilibrium period, control response curves of 1 μM, isoprenaline and ACh were recorded. Force-displacement transducer and Grass model 7 Polygraph were used for recording changes in isometric tension of atria.
5
Evaluating Aortic Vasorelaxation Effects
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Krebs solution in a 20 mL tissue bath was used for conducting the experiment; aortic rings were mounted in it, where bath environment was maintained at 37 °C along with continuous supply of carbogen. Before studying the effect of drug, aortic ring was stabilized by applying tension of 2 g. After the application of PE (1 μM) and K+ (80 mM), vasorelaxant effect of testing material was assessed [37 (link)]. Force-displacement transducer and Grass model 7 Polygraph were used for recording changes in isometric tension of aortic rings.
6
Tracheal Contractility Assay in Mice
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Organ bath studies were performed as previously described23 . Briefly, whole tracheas were dissected out from sacrificed mice and placed in oxygenated Krebs-bicarbonate solution containing (in mM): 118 NaCl, 5.4 KCl, 1 NaH2PO4, 1.2 MgSO4,1.9 CaCl2, 25 NaHCO3, 11.1 dextrose. Tracheas were cleaned of connective tissue and tracheal rings (whole or laterally divided in half), were suspended between two tungsten stirrups in 10 ml organ chambers filled with Krebs solution that was warmed to 37 °C and bubbled with 95% O2–5% CO2 to maintain a pH of 7.4. One stirrup was connected to a strain gauge (model FT03; Grass Instruments, Quincy, MA, USA), and tension was recorded on a Grass Model 7 poly-graph (Grass Instruments). Preparations were stretched to a resting tension of 0.2 g, and washed with fresh Krebs buffer at 15 min intervals during a 60-min equilibration period. After equilibration, tracheas were challenged with either Bam8-22 (10 μM) or electrical field stimulation (12V, 8HZ, 0.5ms, 10s/100s). At the end of each experiment, all tracheas were maximally contracted with carbamylcholine (1mM). The results are expressed as a percentage of maximum contraction.
7
Tracheal Contractility Assay in Mice
8
Tracheal Contractility Assay for Allergic Responses
9
Indirect blood pressure measurement in rats
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The systolic blood pressure (SBP) was monitored indirectly in conscious, pre-warmed rats that were mildly restrained by the tail-cuff method and recorded on a Grass Model 7 polygraph (Grass Instruments Co., Quincy, MA, USA). The rats were trained in the apparatus several times before measurement.
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