The serum glucose, TG, TC, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were measured using a 7020 automatic biochemistry analyzer (Hitachi Ltd., Tokyo, Japan). The serum insulin, LPS, TNF-α and IL6, intestine GLP1, and hepatic PEPCK, G6PC, and GP levels were detected using commercial enzyme-linked immunosorbent assay kits (Cusabio Biotech Co. Ltd., Wuhan, Hubei, China) according to manufacturer’s protocol.
7020 automatic biochemistry analyzer
Manufactured by Hitachi
Sourced in Japan
The 7020 automatic biochemistry analyzer is a laboratory equipment designed for the automated analysis of biochemical samples. It performs various clinical chemistry tests to aid in the diagnosis and monitoring of medical conditions. The 7020 analyzer provides accurate and efficient analysis of a wide range of biochemical parameters, ensuring reliable results for healthcare professionals.
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4 protocols using 7020 automatic biochemistry analyzer
1
Metabolic Biomarker Quantification
2
Serum Biochemistry Analysis Protocol
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The serum biochemical indexes, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bile acid (TBA), alkaline phosphatase (ALP), total bilirubin (T-BIL), and triglyceride (TG), were measured dynamically using a Hitachi 7020 automatic biochemistry analyzer (Tokyo, Japan).
3
Comprehensive Blood Analysis Protocol
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One blood sample (approximately 20 μL) was treated with EDTA-2K for white blood cells (WBC), red blood cells (RBC), haemoglobin (Hb), platelets (PLT), percent of lymphocytes (LY), percent of monocytes (MO), haematocrit (HCT), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean platelet volume (MPV), platelet haematocrit (PCT), platelet distribution width (PDW), and red blood cell distribution width (RDW) using a hematology analyzer MEK-6318K (Nihon Kohden Co., Ltd.). Serum from blood samples collected in separator tubes were measured using a BS-200 automatic biochemistry analyzer (Mindray Co., Ltd.) including aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, nitrogen, creatinine (Cr), total cholesterol (TC), triglyceride (TG), total protein (TP), albumin (Alb), and glucose (Glu). Total calcium (TCa) was measured using the 7020 automatic biochemistry analyzer (Hitachi Co., Ltd.) as well as sodium (Na), potassium (K), and chloride (Cl).
4
Biochemical Profiling of Serum Markers
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Serum levels of UA, BUN, CRE, TG, TC, TBA, HDL, and LDL were measured using a 7,020 automatic biochemistry analyzer (Hitachi Ltd., Tokyo, Japan). The levels of serum LPS, TNF-α, and IL-1β were determined using commercial enzyme-linked immunosorbent assay kits from Cusabio Bio-tech Co. Ltd. (Wuhan, Hubei, China), following the manufacturer’s protocol.
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