Dulbecco’s Modified Eagle’s Medium (DMEM), Roswell Park Memorial Institute 1640 medium (RPMI 1640), fetal bovine serum (FBS), penicillin–streptomycin, and glutamine were purchased from Gibco (Thermo Fisher, Germany). Trypsin-EDTA solution, adenosine, inosine, AMP, ADP, and ATP, the A2b (alloxazine) and A3 adenosine receptors (VUF5574), and caffeine, a non-selective adenosine antagonist, were purchased from Sigma-Aldrich (Germany). The antagonists for A1 (DPCPX) and A2a (SCH-58261) adenosine receptors were purchased from Tocris, UK.
Vuf5574
Manufactured by Merck Group
Sourced in Germany, United States
VUF5574 is a laboratory equipment product. It is designed for general scientific research and analysis applications. The core function of VUF5574 is to provide precise and reliable measurements within a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Adenosine, by Merck Group (1 mentions)
Inosine, by Merck Group (1 mentions)
Dpcpx, by Bio-Techne (1 mentions)
1640 medium rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Merck Group (1 mentions)
Sch58261, by Bio-Techne (1 mentions)
Mrs1754, by Merck Group (1 mentions)
Zm241385, by Merck Group (1 mentions)
Dpcpx, by Merck Group (1 mentions)
2 protocols using vuf5574
1
Adenosine Receptor Activation Assay
2
Adenosine Receptor Antagonist Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four different ligands for specific ARs were tested in this study, including a
selective adenosine A1 receptor (A1R)-specific antagonist
[8-cyclopentyl-1,3-dipropyl xanthine] DPCPX (Sigma, USA, named DPCPXA1R below), a selective adenosine A2A receptor (A2AR)-specific antagonist
[4-(2-[7-amino-2-(2-furyl)[1,2,4]-triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)-phenol]
ZM241385 (Sigma, USA, named ZMA2AR below), a selective adenosine A2B receptor (A2BR)-specific antagonist
[N-(4-cyanophenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1H-purin-8-yl)-
phenoxy]acetamide] MRS1754 (Sigma, USA, named MRSA2BR below), and a selective adenosine A3 receptor (A3R)-specific antagonist
[N-(2-methoxyphenyl)-N′ -[2-(3-pyridinyl)-4-quinazolinyl]-urea] VUF5574 (Sigma,
USA, named VUFA3R below). All reagents were dissolved in 25% dimethylsulfoxide (DMSO)
solution. All other chemicals and solvents were from standard commercial sources
and were of analytical or pharmaceutical grade.
selective adenosine A1 receptor (A1R)-specific antagonist
[8-cyclopentyl-1,3-dipropyl xanthine] DPCPX (Sigma, USA, named DPCPXA1R below), a selective adenosine A2A receptor (A2AR)-specific antagonist
[4-(2-[7-amino-2-(2-furyl)[1,2,4]-triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)-phenol]
ZM241385 (Sigma, USA, named ZMA2AR below), a selective adenosine A2B receptor (A2BR)-specific antagonist
[N-(4-cyanophenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1H-purin-8-yl)-
phenoxy]acetamide] MRS1754 (Sigma, USA, named MRSA2BR below), and a selective adenosine A3 receptor (A3R)-specific antagonist
[N-(2-methoxyphenyl)-N′ -[2-(3-pyridinyl)-4-quinazolinyl]-urea] VUF5574 (Sigma,
USA, named VUFA3R below). All reagents were dissolved in 25% dimethylsulfoxide (DMSO)
solution. All other chemicals and solvents were from standard commercial sources
and were of analytical or pharmaceutical grade.
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