Cells were grown on high-precision borosilicate cover glasses (LH22.1 Roth Labware). For cyclin B staining (1:200; Cell Signalling), cells were fixed in PTEMF buffer or in 4% PFA followed by ice-cold methanol, and stained as previously described [29 (link)]. For γH2AX and phospho-RPA staining, cells were fixed in 4% PFA, permeabilised in 0.5% Triton X-100 in PBS and blocked for one hour in 0.1% Tween and 1% BSA, followed by incubation with primary antibodies: rabbit anti-γH2AX (1:500; Bethyl) or rabbit anti-phospho-RPA S4/S8 (1:500; Bethyl) and subsequent incubation with appropriate secondary antibodies (1:500; Alexa Fluor®).
Rabbit anti phospho rpa s4 s8
Manufactured by Fortis Life Sciences
Rabbit anti-phospho-RPA S4/S8 is a laboratory reagent used to detect the phosphorylation of the S4 and S8 residues of the Replication Protein A (RPA) protein. RPA is a heterotrimeric protein complex that plays a critical role in DNA replication, repair, and recombination. The phosphorylation of RPA at the S4 and S8 sites is associated with DNA damage response pathways.
Automatically generated - may contain errors
Lab products found in correlation
Cyclin b, by Cell Signaling Technology (1 mentions)
Goat anti mouse igg hrp, by Cell Signaling Technology (1 mentions)
Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions)
Mouse anti flag hrp, by Merck Group (1 mentions)
Mouse anti histone h2b, by Abcam (1 mentions)
Rabbit anti rad51, by Abcam (1 mentions)
Rabbit anti nbs1, by Novus Biologicals (1 mentions)
Rabbit anti mdc1, by Abcam (1 mentions)
Mouse anti igg, by Santa Cruz Biotechnology (1 mentions)
Mouse anti vinculin, by Santa Cruz Biotechnology (1 mentions)
Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rabbit anti phospho rpa s4 s8
1
Immunofluorescence Staining of Cell Cycle Markers
2
Immunoprecipitation and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Rabbit-anti-ID3 (Cell Signaling, Cat#9837) and for IP of endogenous ID3 we used agarose beads conjugated with mouse-anti-ID3(Santa Cruz, Cat#sc-56712). Mouse-anti-beta-Actin (Santa Cruz, Cat#sc-47778). Mouse-anti-Vinculin (Santa Cruz, Cat#sc-25336). Mouse-anti-phospho-Histone H2A.X (S139) (Merck, Cat#05-636). Rabbit-anti-phospho-Histone H2A.X (S139) (Abcam, Cat# ab2893). Rabbit-anti-RAD51 (Calbiochem, Cat#PC130). Rabbit-anti-RAD51 (Abcam, Cat#ab176458). Rabbit-anti-XRCC4 (GenTex, Cat#GTX109632). Rabbit-anti-phospho-RPA (S4/S8) (Bethyl, Cat#A300-245A). Mouse-anti-Histone H2B (Abcam, Cat#ab52484). Rabbit-anti-DNA-PKsc (Cell Signaling, Cat#4602). Rabbit-anti-CtIP (Abcam, Cat#70163). Goat-anti-MDC1 (Santa Cruz, Cat#sc-27737). Rabbit-anti-MDC1 (Abcam, Cat#ab11169). Rabbit-anti-NBS1 (Novus Biologicals, Cat#NB100-143). Mouse-anti-RAD50 (Abcam, Cat#ab89). Rabbit-anti-RECQL (Abcam, Cat#ab151501). Mouse-anti-RPA32 (Santa Cruz, Cat#sc-53496). Mouse-anti-Flag-HRP (Sigma, Cat#A8592). Mouse-anti-IgG (Santa Cruz, Cat#sc-2025). Mouse-anti-CenpF (BD bioscience, Cat#610768). Goat-anti-mouse IgG-HRP (Cell Signaling, Cat#7076P2). Goat-anti-rabbit IgG-HRP (Cell Signaling, Cat#7074S). Goat-anti-mouse IgG-AlexaFluor 594 (Molecular Probes, Cat#A11005). Goat-anti-rabbit IgG-AlexaFluor 488 (Molecular Probes, Cat#A11008).
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