Anlotinib hydrochloride capsules (12 mg/capsule) were obtained from Zhengda Tianqing Company (batch number: H20180004). HPLC-grade methanol, ammonium hydroxide, ammonium acetate and acetonitrile were purchased from CNW Technologies. The internal standard, 2-chloro-L-phenylalanine, was obtained from Shanghai Hengbai Biotech Co., Ltd.
Hplc grade methanol
Manufactured by CNW Technologies
Sourced in Germany, United States
HPLC-grade methanol is a high-purity solvent used in high-performance liquid chromatography (HPLC) applications. It serves as a mobile phase component to facilitate the separation and analysis of various chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium hydroxide, by CNW Technologies (2 mentions)
Milli q water purification system, by Merck Group (2 mentions)
Ammonium acetate, by CNW Technologies (2 mentions)
Pyridine, by CNW Technologies (2 mentions)
Mill q water purification system, by Merck Group (1 mentions)
Formic acid, by CNW Technologies (1 mentions)
Ribitol, by Merck Group (1 mentions)
Trypsine, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Alpha minimal essential medium α mem, by Thermo Fisher Scientific (1 mentions)
L glutamine 200 mm, by Thermo Fisher Scientific (1 mentions)
Milli q advantage a10 system, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by CNW Technologies (1 mentions)
13c12 bpa, by Cambridge Isotopes (1 mentions)
Oasis hlb cartridge, by Waters Corporation (1 mentions)
Deionized water, by Thermo Fisher Scientific (1 mentions)
N hexane, by CNW Technologies (1 mentions)
Trichloromethane, by Shanghai Chemical Reagent (1 mentions)
9 protocols using hplc grade methanol
1
Anlotinib Hydrochloride Capsules Analysis
2
Quantification of Ginsenosides and Theophylline
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The standard substances ginsenosides Rg1 (GRg1), Rb1 (GRb1), Rd (GRd), Re (GRe), Rg5 (GRg5), Rk1 (GRk1), notoginsenoside R1 (NGR1) and theophylline (IS) (purity > 99%) were purchased from Shanghai Yuanye Biotechnology Co., Ltd, Shanghai, China. HPLC-grade methanol and formic acid were purchased from CNW Technologies Gmbh (Dusseldorf, Germany) and Sigma-Aldrich (St. Louis, MO), respectively. Ultra-pure water was obtained from a Mill-Q water purification system (Millipore, Burlington, MA). All other reagents were of LC-MS grade.
3
Metabolite Extraction and Derivatization
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HPLC‐grade methanol, chloroform, and pyridine were purchased from CNW Technologies and Adamas Reagent Co., Ltd., respectively. Methoxy amination hydrochloride was purchased from Tokyo Chemical Industry Co., Ltd. Ribitol was purchased from Sigma‐Aldrich trading Co., Ltd. BSTFA (with 1% TMCS, v/v) was purchased from Regis Technologies, Inc. Saturated fatty acid methyl esters (FAMEs) were purchased from Dr. Ehrenstorfer. All other chemicals and reagents used in this research were of analytical grade.
4
Steroid and Phthalate Standards Characterization
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Four steroid standards including E1, E2, E3 and EE2 and BPA were supplied by Sigma-Aldrich (USA). The standards of six phthalates, including DMP, DEP, DBP, BBP, DEHP and DnOP were obtained from Aladdin Reagent Corporation (China). HPLC grade methanol, alkane, acetone, methyl tert-butyl ether (MTBE) and ethyl acetate were supplied by CNW (CNW Technologies GmbH, Germany). Dimethyl sulphoxide (DMSO), sodium hydroxide (NaOH) and a 25% ammonia solution were supplied by Merck (Darmstadt, Germany). Dulbecco's Modified Eagle Medium (DMEM without phenol red (Gibco)), sodium pyruvate (100 mM, sterile-filtered), alpha-Minimal Essential Medium (α-MEM (Gibco)), penicillin-streptomycin, fetal bovine serum (FBS), charcoal-stripped FBS, L-glutamine (200 mM), trypsine (0.5% (Gibco)), phosphate buffered saline (PBS, 1X, pH 7.4) and trypsine without phenol red (10X, 0.5% (Gibco)) were purchased from Life Technologies (United Kingdom). Ultrapure water was obtained using a Milli-Q Advantage A-10 system (Millipore, USA). The physiochemical properties of the investigated steroids, BPA and phthalates are provided in Table S1.
5
Dexamethasone Injection Quantification
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HPLC grade methanol, acetonitrile, ammonium acetate, and ammonium hydroxide were purchased from CNW Technologies (CNW, Germany). All aqueous solutions were prepared with ultrapure water produced by a Milli-Q water purification system (Millipore Corp., Billerica, MA, USA). Dexamethasone solution for injection was purchased from Shanghai Xinyi Pharmaceutical Co (Shanghai, China).
6
BPA Quantification by LC-MS/MS
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The BPA standard was purchased from Aldrich-Sigma (purity >98%, St. Louis, MO, USA). BPA-13 C12 (Cambridge Isotope Laboratories Ins, USA), HPLC-grade methanol, ethyl acetate and ammonium acetate (CNW Technologies) were purchased from Shanghai ANPEL Scientific Instrument Co., Ltd. β-Glucuronidase from Helix pomatia (Type HP-2, aqueous solution, β-glucuronidase activity ≥100000 units/mL, sulfatase activity ≤7500 units/mL) was purchased from Sigma-Aldrich. Deionized water for the preparation of buffers was purified by a Milli-Q ® water-purification system (Millipore, Bedford, MA, USA). An Oasis HLB cartridge (Waters, 200 mg/6 cm 3 , Milford, MA, USA) was purchased from Waters.
Stock solutions of BPA and 13 C12-BPA were prepared at a concentration of 1 mg/mL in methanol. All stock solutions were stored at -20°C. Calibration standards, ranging from 0.20 to 25 μg/L, were prepared from the stock solution through serial dilution by using a methanol-water mixture (50/50, v/v); isotope stock solutions ( 13 C12-BPA, 100 μg/L) were also prepared by using the same methanol-water mixture.
Stock solutions of BPA and 13 C12-BPA were prepared at a concentration of 1 mg/mL in methanol. All stock solutions were stored at -20°C. Calibration standards, ranging from 0.20 to 25 μg/L, were prepared from the stock solution through serial dilution by using a methanol-water mixture (50/50, v/v); isotope stock solutions ( 13 C12-BPA, 100 μg/L) were also prepared by using the same methanol-water mixture.
7
Metabolomic Profiling using GC-MS
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Pyridine and chloroform were purchased from Admas (Shanghai, China). Bistrifluoroacetamide (BSTFA) and 1% trimethyl chloro silane (TMCS, v/v) were from REGIS Technologies Inc (Morton Grove, IL, USA) and methanol (HPLC grade) from CNW Technologies (Shanghai, China). L-2-Chlorophenylalanine was from Hengbai Biotechnology Co Ltd (Shanghai, China). Methoxyamine hydrochloride was from TCI company (Shanghai, China). Deionized water (Thermo; Waltham, MA, USA) was used throughout the experiment.
8
Plasma Metabolite Extraction and Derivatization
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An aliquot of 50 µL plasma was mixed with 200 µL of methanol (HPLC grade; CNW Technologies, Duesseldorf, Germany) containing 25 µmol/L 2-chloro-L-phenylalanine (Shanghai Hengbai Biotech, Shanghai, China) as an internal standard, which was used for normalization. After vortexing for 30 s, samples were ultra-sonicated in iced water for 10 min. After centrifugation at 12 000 rpm at 4 °C for 15 min, 180 µL of supernatant for each sample were transferred into a new tube and dried in a vacuum concentrator. The residues of each sample were then added with 20 µL of 20 mg/mL methoxyamine hydrochloride (AR grade; TCI, OR, USA) in pyridine (HPLC grade; Adamas Pharmaceuticals, CA, USA). The above samples were kept at 80 °C for 30 min, after which 40 µL of bis-(trimethylsilyl) trifluoroacetamide (BSTFA) in 1% TMCS (REGIS Technologies, IL, USA) was added and samples were incubated at 70 °C for 1.5 h. The samples were stored at room temperature until analysis. Quality control (QC) samples were prepared by pooling an aliquot of 30 µL from each sample.
9
Metabolite Extraction and Derivatization
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All chemicals and solvents were of analytical or HPLC grade. Water, methanol (HPLC grade), acetonitrile (UHPLC grade), pyridine, n-hexane, methoxylamine hydrochloride (97%), N-methyl-N-trimethylsilyltrifluoroacetamid (MSTFA) with 1% trimethylchlorosilane (TMCS) were purchased from CNW Technologies GmbH (Düsseldorf, Germany). Trichloromethane was from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China). 2-chloro-l-phenylalanine was purchased from Shanghai Hengchuang Bio-technology Co., Ltd. (Shanghai, China).
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