Trizol reagent and primers

DMSO (dimethyl sulfoxide) and Hoechst dye 33,258 for nucleus staining were purchased from Sigma-Aldrich (St. Louis, USA). Daphnetin (7,8-Dihydroxycoumarin, CAS 486–35-1) was obtained from PharmaBlock Sciences (Nanjing, China) and dissolved in DMSO. Carbopol 940 and azone were purchased from Rhawn (Shanghai, China). IMQ cream was obtained from Sichuan Med-Shine Pharmaceutical Co., LTD (Sichuan, China). IL-17A, IL-22, oncostatin M, IL-1α, and TNF-α cytokines were purchased from PeproTech (RockyHill, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). TRIzol™ reagent and primers were obtained from Invitrogen (Carlsbad, USA). RevertAid First Strand cDNA Synthesis Kit was purchased from Thermo Fisher Scientific (USA). SYBR Green PCR Master Mix kit was purchased from TaKaRa (Japan). Rabbit anti-p65, anti-p-p65 (Ser536), anti-β-actin primary antibodies and Alexa Fluor 555-labeled anti-rabbit secondary antibody were purchased from Cell Signaling Technology (Beverly, USA).