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Sodium periodate

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Sodium periodate is a chemical compound commonly used in laboratory settings. It functions as an oxidizing agent, capable of cleaving carbon-carbon bonds in certain organic molecules. This process is often employed in analytical techniques and sample preparation procedures.

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Lab products found in correlation

Sequencing grade trypsin, by Promega (4 mentions) Pngase f, by New England Biolabs (3 mentions) Hydrazide resin, by Bio-Rad (2 mentions) Iodoacetamide, by Merck Group (2 mentions) Urea, by Merck Group (2 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (2 mentions) Tris 2 carboxyethyl phosphine, by Merck Group (2 mentions) Orbitrap velos mass spectrometer, by Thermo Fisher Scientific (1 mentions) 2d nano lc system, by Thermo Fisher Scientific (1 mentions) Magic c18 packing material, by Bruker (1 mentions) Sambucus nigra lectin, by Vector Laboratories (1 mentions) Sep pak c18 1 cc vac cartridges, by Waters Corporation (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Acetonitrile, by Waters Corporation (1 mentions) Itraq reagent, by AB Sciex (1 mentions) Sep pak c18 1cc vac cartridge, by Waters Corporation (1 mentions) 5 m nacl, by Merck Group (1 mentions) Ammonia bicarbonate, by Merck Group (1 mentions) Sodium sulphite, by Merck Group (1 mentions)

5 protocols using sodium periodate

1

Glycoprotein Enrichment and Analysis

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Aleuria aurantia lectin (AAL)-, Sambucus nigra lectin (SNA)-, and wheat germ agglutinin (WGA)-conjugated agarose beads were purchased from Vector Laboratories (Burlingame, CA). Tris (2-carboxythyl) phosphine (TCEP) was purchased from Pierce (Rockford, IL). Sequencing-grade trypsin was from Promega (Madison, WI). Peptide-N-glycosidase F (PNGase) was from ProZyme (San Leandro, CA). Sodium periodate, hydrazide resin, and P6 desalting spin columns were from Bio-Rad (Hercules, CA). C18 and MCX desalting columns were from Waters (Milford, MS). The high-performance liquid chromatography–mass spectrometry (HPLC–MS) platform used in this study includes an Eksigent 2D nanoLC system (Dublin, CA) and a Thermo Scientific Orbitrap Velos mass spectrometer (Waltham, MA). Magic C-18 packing material was from Michrom Bioresources (Auburn, CA), and all other chemicals were purchased from Sigma (St. Louis, MO).
Li Y., Shah P., De Marzo A.M., Van Eyk J.E., Li Q., Chan D.W, & Zhang H. (2015). Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. Analytical chemistry, 87(9), 4683-4687.
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2

Comprehensive Protein Assay Protocol

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The bicinchoninic acid (BCA) protein assay kit was purchased from Pierce (Rockford, IL); hydrazide support and sodium periodate were purchased from Bio-Rad (Hercules, CA); sequencing-grade trypsin was purchased from Promega (Madison, WI); PNGase F was purchased from New England Biolabs (Ipswich, MA); Sep-Pak C18 1 cc Vac Cartridges were purchased from Waters (Milford, MA); and acetonitrile (ACN), trifluoroacetic acid (TFA), formic acid, urea, tris(2-carboxyethyl)phosphine (TCEP), iodoacetamide, phorbol 12-myristate 13-acetate (PMA), 5 M NaCl, sodium dodecyl sulfate (SDS), 10× phosphate buffered saline buffer (PBS) pH 7.4 and 1 M Tris–HCl pH 8.0 buffer were purchased from Sigma-Aldrich (St. Louis, MO).
Yang W., Zhou J.Y., Chen L., Ao M., Sun S., Aiyetan P., Simmons A., Zhang H, & Jackson J.B. (2014). Glycoproteomic analysis identifies human glycoproteins secreted from HIV latently infected T cells and reveals their presence in HIV+ plasma. Clinical proteomics, 11(1), 9.
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3

Glycoprotein Analysis Using Hydrazide Resin

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Hydrazide resin (Bio-Rad, Hercules, CA), sodium periodate (Bio-Rad, Hercules, CA), tris (2-carboxyethyl) phosphine (TCEP) (Pierce, Rockford, IL), PNGase F (New England Biolabs, Ipswich, MA), sequencing grade trypsin (Promega, Madison, WI), C18 columns (Waters, Sep-Pak Vac) were used in our experiment. Additional lab supply and chemicals were purchased from Sigma-Aldrich. iTRAQ reagent and mass calibration standards were from AB SCIEX (Foster City, CA); BCA assay kit was from Pierce (Rockford, IL).
Li Q.K., Shah P., Tian Y., Hu Y., Roden R.B., Zhang H, & Chan D.W. (2017). An integrated proteomic and glycoproteomic approach uncovers differences in glycosylation occupancy from benign and malignant epithelial ovarian tumors. Clinical Proteomics, 14, 16.
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4

Glycoprotein Enrichment and Identification

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Sodium periodate and hydrazide beads were purchased from Bio-Rad (Hercules, CA); bicinchoninic acid protein assay (BCA) assay kit was purchased from Pierce (Rockford, IL); sequencing-grade trypsin was from Promega (Madison, WI); Sep-Pak C18 1cc Vac Cartridge was from Waters (Milford, MA); PNGase F was from New England Biolabs (Ipswich, MA); Other chemicals such as urea, ammonia bicarbonate (AB), ACN, TFA, tris (2-carboxyethyl) phosphine (TCEP), iodoacetamide, 5 M NaCl, sodium sulphite and phorbol 12-myristate 13-acetate (PMA) were purchased from Sigma-Aldrich (St. Louis, MO).
Yang W., Jackson B, & Zhang H. (2016). Identification of glycoproteins associated with HIV latently infected cells using quantitative glycoproteomics. Proteomics, 16(13), 1872-1880.
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5

Lectin Microarray Analysis of CEA

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Lectin microarrays were purchased from BCBIO (Guangzhou, China). Commercial standard Carcinoembryonic Antigen (CEA) was purchased from LEE BioSolutions, Inc (St. Louis, MO). Human Carcinoembryonic Antigen (CEA) ELISA Kit was purchased from Yu Ping biotechnology (Shanghai, China). Anti-Human CEA antibody was purchased from eBioscience Inc (San Diego, CA). Rabbit anti-mouse IgG-Alexa Fluor 647 conjugate was purchased form Invitrogen (Eugene, OR). The incubation chamber and holder for the lectin microarray were purchased from Whatman Schleicher and Schuell (Keene, NH). Sodium periodate was purchased from Bio-Rad Laboratories (Hercules, CA). 4-(4-N-maleimidophenyl) butyric acid hydrazide hydrochloride (MPBH) was from Thermo Fisher Scientific, Inc. (Rockford, IL). All other chemicals and reagents were purchased from Sigma-Aldrich (St.Louis, MO).
Zhao Q., Zhan T., Deng Z., Li Q., Liu Y., Yang S., Ji D, & Li Y. (2018). Glycan analysis of colorectal cancer samples reveals stage-dependent changes in CEA glycosylation patterns. Clinical Proteomics, 15, 9.
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