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2 protocols using ammonium carbonate nh4 2co3

1

Formulation and Characterization of Lipid Nanoparticles

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Ammonium carbonate ((NH4)2CO3) and anhydrous calcium chloride (CaCl2) were purchased from Sinopharm Chemical Reagent Co, Ltd (Shanghai, China). MIT was a gift from Dalian Meilun Biotech Co, Ltd (Dalian, China). PL (S100) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy[polyethylene glycol]-2000) (DSPE-PEG2000) were obtained from Lipoid GmbH (Ludwigshafen, German). DSPE-PEG-FA (35,000 Da) was obtained from Ponsure Biotechnology (Shanghai, China). LysoTracker Blue, Fluo-4 AM, fluorescein diacetate (FDA), propidium iodide (PI), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanine iodide (DiR) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and reagents otherwise stated were from Sinopharm Chemical Reagent Co, Ltd and of analytical grade.
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2

Bioactive Compounds from Dendrobium officinale

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The Dendrobium officinale (DO) under the forest for 3–4-year-old was collected in Wenshan County, Kunming City, Yunnan Province, China, in June 2021.
Chemicals and reagents such as potassium chloride (KCl), dipotassium hydrogen phosphate (K2HPO4), magnesium chloride hexahydrate (MgCl26H2O), sodium chloride (NaCl), hydrochloric acid (HCl), and ammonium carbonate (NH4)2CO3, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), vitamin C (Vc), and 2,6-di-tert-butyl-4-methylphenol (BHT) were purchased from Sinopharm Chemical Reagent (Beijing, China). Acetonitrile (chromatographic grade) was purchased from Merck (Darmstadt, Germany). Other reference standards were supplied by Yuanye Bio-Technology (Shanghai, China). The pancreatin (from porcine gastric mucosa), and pepsin (from porcine gastric mucosa) were from Ruijin Medical Devices Co. Ltd (Jiangxi, China). The α-glucosidase (from Saccharomyces cerevisiae), α-amylase (from hog pancreas), acarbose, P-nitrophenyl-α-D-glucopyranoside (pNPG), and short-chain fatty acids standards (acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, and caproic acid) were purchased from Sigma-Aldrich (St.Louis, MO, USA).
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