Waters lc ms system

Manufactured by Waters Corporation

The Waters LC-MS system is a combination of a liquid chromatography (LC) instrument and a mass spectrometry (MS) detector. It is used for the separation, identification, and quantification of chemical compounds in a sample. The LC-MS system provides high-resolution separation and precise mass analysis, making it a versatile tool for a wide range of analytical applications.

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LC-MS system (30 citations)

4 protocols using waters lc ms system

Radiolabeled Compound Synthesis and Purification

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O-tolidine and 1-amino-naphthol-2,4-disulfonic acid monosodium salt were purchased from TCI America (Portland, OR) and NOTA-NHS was obtained from Marcocyclics (Dallas, TX). All other chemicals were purchased from Sigma-Aldrich. Waters 600 high-performance liquid chromatography (HPLC) system with a Waters 996 Photodiode Array Detector (PDA) and an online radioactivity detector (Beckman) using a semi-preparative C₁₈ HPLC column (XTerra Prep RP18, 10 µm, 7.8 x 300 mm, Waters) was used for the purification of products. Varian BOND ELUT C₁₈ column (100 mg) was used for solid phase extraction. A Perkin-Elmer 200 series HPLC pump with a Waters 2487 UV detector and a Bioscan Flow-Count detector using an analytical C₁₈ HPLC column (XTerra 5 µm, 150 x 4.6 mm, Waters) was used for analysis of labeled compounds. HPLC runs a linear gradient starting from 5% A (0.1% TFA in acetonitrile) and 95% B (0.1% TFA in water) for 5 min and increasing to 65% A at 35 min with a flow rate of 5 ml/min for semi-prep HPLC and 1 ml/min for analytical HPLC. Mass spectra were obtained with Waters LC-MS system (Waters, Milford, MA) that includes an Acquity UPLC system coupled to the Waters Q-Tof Premier high-resolution mass spectrometer. The ¹⁸F-fluoride was obtained from NIH cyclotron facility.

Chen H., Wang G., Lang L., Jacobson O., Kiesewetter D.O., Liu Y., Ma Y., Zhang X., Wu H., Zhu L., Niu G, & Chen X. (2016). Chemical Conjugation of Evans Blue Derivative: A Strategy to Develop Long-Acting Therapeutics through Albumin Binding. Theranostics, 6(2), 243-253.

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Peptide Purification and Characterization

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Waters 600 high-performance liquid chromatography (HPLC) system with a Waters 996 Photodiode Array Detector (PDA) using a preparative C₁₈ HPLC column (PROTO 300 C₁₈ 5 μm, 250 x 20 mm, Higgins Analytical, Inc.) was used for peptide purification. The peptides were analyzed using a Perkin-Elmer 200 series HPLC pump with a Waters 2487 UV detector and an analytical C₁₈ HPLC column (Waters Symmetry C₁₈ 5 μm, 150 x 3.9 mm). Mass spectra were obtained with a Waters LC-MS system (Waters, Milford, MA) that included an Acquity UPLC system coupled to a Waters Q-Tof Premier high-resolution mass spectrometer. All chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise stated in the procedure.

Li D., Zhang J., Chi C., Xiao X., Wang J., Lang L., Ali I., Niu G., Zhang L., Tian J., Ji N., Zhu Z, & Chen X. (2018). First-in-human study of PET and optical dual-modality image-guided surgery in glioblastoma using 68Ga-IRDye800CW-BBN. Theranostics, 8(9), 2508-2520.

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Analytical Characterization of Synthetic Compounds

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Starting materials and other reagents were purchased from commercial suppliers and were used without further purification unless otherwise noted. All reactions were monitored by thin layer chromatography (TLC) with 0.25 mm E. Merck pre-coated silica gel plates (60 F254) and Waters LCMS system (Waters 2489 UV/Visible Detector, Waters 3100 Mass, Waters 515 HPLC pump, Waters 2545 Binary Gradient Module, Waters Reagent Manager, Waters 2767 Sample Manager) using SunFireTM C18 column (4.6 × 50 mm, 5 μm particle size): solvent gradient = 100% A at 0 min, 1% A at 5 min; solvent A = 0.035% TFA in Water; solvent B = 0.035% TFA in CH3CN; flow rate : 2.5 mL/min. Purification of reaction products was carried out by flash chromatography using CombiFlash®Rf with Teledyne Isco RediSep®Rf High Performance Gold or Silicycle SiliaSepTM High Performance columns (4 g, 12 g, 24 g, 40 g, 80 g, or 120 g). The purity of all compounds was over 95% and was analyzed with Waters LCMS system. ¹H NMR and ¹³C NMR spectra were obtained using a Varian Inova-400 (400 MHz for 1H, and 75 MHz for 13C) spectrometer. Chemical shifts are reported relative to chloroform (δ = 7.24) for ¹H NMR or dimethyl sulfoxide (δ = 2.50) for ¹H NMR and dimethyl sulfoxide (δ = 39.51) for ¹³C NMR. Data are reported as (br = broad, s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet).

Hatcher J.M., Bahcall M., Choi H.G., Gao Y., Sim T., George R., Jänne P.A, & Gray N.S. (2015). Discovery of inhibitors that overcome the G1202R ALK Resistance Mutation. Journal of medicinal chemistry, 58(23), 9296-9308.

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Quantitative Analysis of Rapamycin in Tissues

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For quantitative analysis of the concentration of rapamycin, the tumors and major organs as well as the plasma at the indicated time points were harvested and subjected to LC-MS analysis, which employed a Waters LC-MS system (Waters, Milford, MA) including an Acquity UPLC system coupled to the Waters Q-Tof Premier high-resolution mass spectrometer as described previously^{22 (link)}. Briefly, An Acquity BEH Shield RP18 column (150 mm × 2.1 mm) was eluted with a two-solution gradient of solution A (2 mM ammonium formate, 0.1% formic acid, and 5% CH₃CN) and solution B (2 mM ammonium formate and 0.1% formic acid in CH₃CN). The elution profile, at 0.2 mL/min, had the following components: initial condition at 100% (v:v) A and 0% B; gradient 0–40% B over 15 min; isocratic elution at 40% B for an additional 3 min; 40–80% B over 2 min; and re-equilibrated with A for an additional 4 min. For quantitative analysis of the concentration of rapamycin, an LC/MS system consisting of an Agilent 1200 autosampler, Agilent 1200 LC pump and an AB/MDS Sciex 4000 Q-TRAP (AB Sciex, Foster City, CA, USA). Standards were prepared for rapamycin covering the concentration range from 0.001 to 1μM in 1:1 CH₃CN-water. Three replicate injections (10 μL) were made for each concentration level.

Wang F., Yang K., Wang Z., Ma Y., Gutkind J.S., Hida N., Niu G, & Tian J. (2016). Combined image guided monitoring the pharmacokinetics of rapamycin loaded human serum albumin nanoparticles with a split luciferase reporter. Nanoscale, 8(7), 3991-4000.

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