Antiserum against a peptide corresponding to amino acids 25–39 of human CHMP6 was raised in two rabbits and affinity purified on Affi-Gel beads containing immobilized peptide. Rabbit anti-CHMP3, rabbit anti-ALIX, rabbit anti-EAP30, rabbit anti-CHMP4B, and rabbit anti-EAP20 were described previously (Sharma et al., 2004 (link); Cabezas et al., 2005 (link); Bache et al., 2006 (link); Malerød et al., 2007 (link); Sagona et al., 2010 (link)). Mouse anti–α-tubulin (Sigma-Aldrich), sheep anti–α-tubulin (Cytoskeleton), mouse anti–β-actin (Sigma-Aldrich), goat anti-RacGAP1 (Abcam), rabbit anti-MKLP1 (Santa Cruz Biotechnology, Inc.), goat anti-V5 (Abcam), mouse anti-GFP (Roche), mouse anti-TSG101 (BD Transduction Laboratories), rabbit anti-CHMP6 (Santa Cruz Biotechnology, Inc.), rabbit anti-CEP55 (Abnova), rabbit anti-VPS28 (Santa Cruz Biotechnology, Inc.), rabbit anti-IST1 (Proteintech), rabbit anti-CHMP4A (Santa Cruz Biotechnology, Inc.), and rabbit anti-mCherry (Acris) were used as primary antibodies. Secondary antibodies included anti-mouse, anti-rabbit, and anti-goat Alexa Fluor 488 (Jackson ImmunoResearch), Alexa Fluor 555 (Molecular Probes), Alexa Fluor 568 (Molecular Probes), Alexa Fluor 647 (Jackson ImmunoResearch), and DyLight649 (Jackson ImmunoResearch).
Rabbit anti vps28
Manufactured by Santa Cruz Biotechnology
Rabbit anti-VPS28 is an antibody product developed by Santa Cruz Biotechnology. It is designed to detect the VPS28 protein, which is a component of the ESCRT-I complex involved in the sorting of ubiquitinated proteins into multivesicular bodies.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β actin, by Merck Group (2 mentions)
Mouse anti tsg101, by BD (1 mentions)
Mouse anti gfp, by Roche (1 mentions)
Mouse anti α tubulin, by Merck Group (1 mentions)
Alexa fluor 555, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568, by Thermo Fisher Scientific (1 mentions)
Dylight 649, by Jackson ImmunoResearch (1 mentions)
Alexa fluor 488, by Jackson ImmunoResearch (1 mentions)
Alexa fluor 647, by Jackson ImmunoResearch (1 mentions)
Mouse anti tsg101, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti cd63, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti eap30, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti eap45, by Santa Cruz Biotechnology (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
2 protocols using rabbit anti vps28
1
Antibody Production and Characterization
2
Immunoprecipitation of HBV Virions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For immunoprecipitation of HBV virions, a mixture of rabbit antibodies against the L and S envelope proteins was used as described [26] (link). Polyclonal antisera against recombinant native (K45) or denatured (K46) core particles were raised in rabbits [33] (link). The MA18/7 mouse antibody recognizing the HBV L protein was a gift from K.-H. Heermann (University of Göttingen, Germany). Commercially available antibodies were as follows: mouse anti-β-actin (Sigma-Aldrich), rabbit anti-CD63 (Santa Cruz Biotechnology), mouse anti-EAP20 (VPS25, Santa Cruz Biotechnology), rabbit anti-EAP30 (Santa Cruz Biotechnology), rabbit anti-EAP45 (Santa Cruz Biotechnology), mouse anti-FLAG (Sigma-Aldrich), mouse anti-Tsg101 (Santa Cruz Biotechnology), and rabbit anti-Vps28 (Santa Cruz Biotechnology). Peroxidase-labeled, secondary antibodies were obtained from Dianova, and fluorophor-labeled antibodies were purchased from Molecular Probes.
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