Panel 1C was acquired with a ZEISS LSM 880 with Airyscan system equipped with a Plan-Apochromat 63×/1.40 Oil DIC M27 objective and using 488 and 561 nm wavelength lasers. Panels 2A,B S3A, S6A and S7B were acquired with a DeltaVision PersonalDV (Applied Precision) with either a Plan Apo 60x/1.42 NA or a UPlanSApo 100x/1.4 NA oil lenses. Panels 2F, 3B,H, S3B,C, S5 and S7A were acquired on a Leica TCS SP5 confocal microscope equipped with a HCX PL APO CS 63.0x/1.40 NA oil lens. FRET experiments were performed on HeLa cells using the Leica FRET AB wizard. Ku70(AF488)/Ku80(488 DyLight)/DNA-PKcs(488 DyLight) served as donors and LMNA (AF594 for Ku80 and DNA-PKcs FRET; AF555 for Ku70 FRET) as acceptor. Panels 3C,F were acquired with a OMX Structured Illumination Super-resolution Scope equipped with a PlanApo 60x/1.40 Oil DIC objective and using 488 and 568 nm wavelength lasers.
Plan apo 60x 1.42 na
Manufactured by Cytiva
The Plan Apo 60x/1.42 NA is a high-quality objective lens for use in advanced microscopy applications. It provides a numerical aperture of 1.42, enabling high-resolution imaging. The lens is designed using a Plan Apo optical configuration, which helps to ensure flat field and high contrast performance.
Automatically generated - may contain errors
Lab products found in correlation
Deltavision personaldv, by Cytiva (2 mentions)
Lsm 880 with airyscan system, by Zeiss (2 mentions)
Uplansapo 100x 1.4 na, by Cytiva (2 mentions)
Hcx pl apo cs 63.0x 1 40 na oil lens, by Leica (2 mentions)
Fret ab wizard, by Leica (2 mentions)
Tcs sp5 confocal microscope, by Leica (2 mentions)
Plan apochromat 63 1.40 oil dic m27 objective, by Zeiss (2 mentions)
Deltavision core imaging system, by Cytiva (2 mentions)
Softworx, by Cytiva (1 mentions)
Coolsnap hq2 camera, by Cytiva (1 mentions)
4 protocols using plan apo 60x 1.42 na
1
Multimodal Microscopy Techniques for DNA Repair
2
Multimodal Microscopy Techniques for DNA Repair
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Panel 1C was acquired with a ZEISS LSM 880 with Airyscan system equipped with a Plan-Apochromat 63×/1.40 Oil DIC M27 objective and using 488 and 561 nm wavelength lasers. Panels 2A,B S3A, S6A and S7B were acquired with a DeltaVision PersonalDV (Applied Precision) with either a Plan Apo 60x/1.42 NA or a UPlanSApo 100x/1.4 NA oil lenses. Panels 2F, 3B,H, S3B,C, S5 and S7A were acquired on a Leica TCS SP5 confocal microscope equipped with a HCX PL APO CS 63.0x/1.40 NA oil lens. FRET experiments were performed on HeLa cells using the Leica FRET AB wizard. Ku70(AF488)/Ku80(488 DyLight)/DNA-PKcs(488 DyLight) served as donors and LMNA (AF594 for Ku80 and DNA-PKcs FRET; AF555 for Ku70 FRET) as acceptor. Panels 3C,F were acquired with a OMX Structured Illumination Super-resolution Scope equipped with a PlanApo 60x/1.40 Oil DIC objective and using 488 and 568 nm wavelength lasers.
3
Fluorescent Imaging of Immobilized Nematodes
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Animals were washed and immobilized in M9 solution containing 50 mM sodium azide and mounted on 2% agarose pads containing 50 mM sodium azide. Image stacks were collected on a DeltaVision Core imaging system (Applied Precision) with a UApo 40x/1.35 NA, PlanApo 60x/1.42 NA, or UPlanSApo 100x/1.40 NA oil immersion objective and a CoolSnap HQ2 camera. Images were deconvolved using Softworx (Applied Precision) and maximum intensity projections were generated in ImageJ (Fiji) (Schindelin et al., 2012 (link)). The brightness and contrast of each projection were linearly adjusted in Affinity Photo 1.10.1. Fluorescent signals were pseudo-colored, and merged images were generated using the Screen layer mode in Affinity Photo 1.10.1.
4
Glial Cell Marker Consistency Analysis
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