Mouse anti ctnt

Cells were fixed with 4% paraformaldehyde, permeabilized in 0.3% Triton X-100/PBS and incubated with primary antibodies overnight at 4°C at following dilutions: mouse anti-cTnT (1:200, Lab Vision), mouse anti-MF-20 (1:20 Developmental Studies Hybridoma Bank), rabbit anti-MLC2v (1:200, ProteinTech Group), mouse anti-alpha-Actinin (1:200, Sigma-Aldrich). Cells were then washed and incubated with Alex Fluor 488 goat anti-mouse IgG (1:500, Life Technologies) and/or Alex Fluor 568 goat anti-rabbit IgG (1:500, Life Technologies) for 1 h at RT. Cell nuclei were counter stained with DAPI (1 μg/mL, Sigma-Aldrich). Images were captured using a Zeiss LSM780 confocal microscope, and were analyzed in ZEN 2011 software.

Flow cytometry analyses were performed as described previously (Zhang et al., 2012 (link)), with antibody concentrations as following: mouse anti-cTnT (1:200, Lab Vision), mouse anti-MF-20 (1:20 Developmental Studies Hybridoma Bank), and Alex Fluor 488 goat anti-mouse IgG (1:500, Life Technologies). Data were collected and analyzed on a LSRII flow cytometer (Becton-Dickinson).