Gonococcal base agar was made from 10 g/l dehydrated agar (BD Biosciences, Bedford, MA), 5 g/l NaCl (Roth, Darmstadt, Germany), 4 g/l K2HPO4 (Roth), 1 g/l KH2PO4 (Roth), 15 g/l Proteose Peptone No. 3 (BD Biosciences), 0.5 g/l soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/l D-glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL x H2O (Roth), 1 mg/l thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/l vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch.
Dehydrated agar
Manufactured by BD
Sourced in United States
Dehydrated agar is a powdered form of agar, a polysaccharide derived from red algae. It is a gelling agent commonly used in microbiological media to support the growth of various microorganisms in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Soluble starch, by Merck Group (4 mentions)
Thiamine pyrophosphate, by Merck Group (4 mentions)
D glucose, by Carl Roth (4 mentions)
L glutamine, by Carl Roth (4 mentions)
4 aminobenzoic acid, by Merck Group (4 mentions)
Vitamin b12, by Merck Group (4 mentions)
Proteose peptone no 3, by BD (4 mentions)
K2hpo4, by Carl Roth (3 mentions)
Kh2po4, by Carl Roth (3 mentions)
Sodium chloride (nacl), by Carl Roth (3 mentions)
Fe no3 3, by Merck Group (3 mentions)
Thiamine hcl, by Carl Roth (3 mentions)
β nicotinamide adenine dinucleotide, by Carl Roth (3 mentions)
Isovitalex, by Carl Roth (2 mentions)
L cysteine hcl x h2o, by Carl Roth (2 mentions)
L cysteine hcl h2o, by Carl Roth (2 mentions)
Thiamine hcl, by Merck Group (1 mentions)
Kh2po4, by BD (1 mentions)
Isovitalex, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide, by Merck Group (1 mentions)
4 protocols using dehydrated agar
1
Gonococcal Base Agar Composition
2
Gonococcal Base Agar Preparation
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Media composition, assay preparation, and setup follow [27 (link)]. Gonococcal base agar was made from 10 g/L dehydrated agar (BD Biosciences, Bedford, Massachusetts, United States of America), 5 g/L NaCl (Roth, Darmstadt, Germany), 4 g/L K2HPO4 (Roth), 1 g/L KH2PO4 (Roth), 15 g/L Proteose Peptone No. 3 (BD Biosciences), 0.5 g/L soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/L D-glucose (Roth), 0.1 g/L L-glutamine (Roth), 0.289 g/L L-cysteine-HCL × H2O (Roth), 1 mg/L thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/LFe(NO3) 3 (Sigma-Aldrich), 0.03 mg/L thiamine HCl (Roth), 0.13 mg/L 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/L β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/L vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch. Employed bacterial strains (Table i in S1 Text ) are derivatives of strain N. gonorrhoeae MS11.
3
Gonococcal Growth Media Formulation
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Gonococcal (GC) base agar was made from 10 g/l dehydrated agar (BD Biosciences), 5 g/l NaCl (Roth), 4 g/l K2HPO4 (Roth), 1 g/l KH2PO4 (Roth), 15 g/l Proteose Peptone No. 3 (BD Biosciences), 0.5 g/l soluble starch (Sigma-Aldrich) and supplemented with 1% IsoVitaleX (IVX). IVX was made from 1 g/l D-glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL x H2O (Roth), 1 mg/l thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/l thiamine HCL (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/l vitamin B12 (Sigma-Aldrich). GC liquid medium is identical to the base agar composition but lacks both agar and starch.
4
Preparation of GC-base Agar Medium
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GC base agar was made from 10 g/L dehydrated agar (BD Biosciences, Bedford, MA), 5 g/L NaCl (Roth, Darmstadt, Germany), 4 g/L K2HPO4 (Roth), 1 g/L KH2PO4 (Roth), 15 g/L Proteose Peptone No. 3 (BD Biosciences), 0.5 g/L soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/L D-glucose (Roth), 0.1 g/L L-glutamine (Roth), 0.289 g/L l -cysteine-HCL × H2O (Roth), 1 mg/L thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/L Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/L thiamine HCl (Roth), 0.13 mg/L 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/L β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/L vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch.
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