Model tm 1000 sem

Manufactured by Hitachi

Sourced in Japan

The Hitachi Model TM-1000 SEM is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It operates at an accelerating voltage of 15 kV and can achieve a magnification range of up to 10,000x. The TM-1000 SEM provides a compact and user-friendly solution for researchers and technicians requiring detailed surface characterization and morphological information.

Automatically generated - may contain errors

Lab products found in correlation

Model hcp 2, by Hitachi (3 mentions) E 1010, by Hitachi (2 mentions) Model h 7650, by Hitachi (2 mentions) Em uc7 ultratome, by Leica (1 mentions) Model h 7650 tem, by Hitachi (1 mentions)

6 protocols using model tm 1000 sem

Skin Sample Preparation for SEM Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Skin samples adopted for the experiment refer to 2.7. After treated by different formulations for 24 h, the skin samples were treated as follows:
Double fixation: The samples were cut into 1 cm × 1 cm size and first fixed with 2.5% glutaraldhyde in phosphate buffer (0.1 M, pH7.0) for more than 4 h; washed three times in the phosphate buffer (0.1 M, pH7.0) for 15 min at each step; then postfixed with 1% O_SO₄ in phosphate buffer for 1–2 h and washed three times in the phosphate buffer (0.1 M, pH7.0) for 15 min at each step. Next, dehydration: the sample was first dehydrated by a graded series of ethanol (30%,50%,70%,80%,90%,95% and 100%) for about 15 to 20 min at each step, transferred to the mixture of ethanol and iso-amyl acetate (v:v = 1:1) for about 30 min, then transferred to pure iso-amyl acetate for about 1 h or overnight. In the end, the sample was dehydrated in Hitachi Model HCP-2 critical point dryer with liquid CO₂. Finally, coating and observation: the dehydrated sample was coated with gold-palladium in Hitachi Model E-1010 ion sputter for 4–5 min and observed in Hitachi Model TM-1000 SEM.

Niu X.Q., Zhang D.P., Bian Q., Feng X.F., Li H., Rao Y.F., Shen Y.M., Geng F.N., Yuan A.R., Ying X.Y, & Gao J.Q. (2019). Mechanism investigation of ethosomes transdermal permeation. International Journal of Pharmaceutics: X, 1, 100027.

+ Open protocol

+ Expand

Imaging Plant Reproductive Structures with SEM and TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

For scanning electron microscopy (SEM), anthers at the anthesis stage and mature pollen grains were spread, coated with gold–palladium in an Eiko Model ion coater (Ibaraki, Japan) for 5 min, and then observed in a Hitachi Model TM-1000 SEM (Tokyo, Japan). For transmission electron microscopy (TEM), anthers were fixed and embedded as described above. Ultrathin sections (70 nm) were obtained using an ultramicrotome (Reichert-Jung Ultracut E, Vienna, Austria) and stained with uranyl acetate and alkaline lead citrate for 15 min. Images were recorded with a Hitachi Model H-7650 TEM (Tokyo, Japan).

Miao Y., Cao J., Huang L., Yu Y, & Lin S. (2021). FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis. BMC Plant Biology, 21, 254.

+ Open protocol

+ Expand

Morphological Analysis of Fungal Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fungi were cultured at 30,000 cells/mL, Berberine hydrochloride (1 mg/mL) and Clotrimazole (0.5 μg/mL) were added in fungi for 6 h and the fungi were analyzed as well as DMSO controls. Berberine hydrochloride and clotrimazole were dissolved in DMSO, then dissolved in distilled water, the concentration of DMSO in water was 5%.
For TEM, the samples were fixed with 2.5% glutaraldehyde in phosphate buffer (0.1 M, pH 7.0; >4 h) followed by post-fixation with 1% OsO₄ in phosphate buffer (1–2 h). After washing, the specimens were dehydrated by graded ethanol and incubated in acetone. After resin embedding, the samples were sectioned on a LEICA EM UC7 ultratome, followed by staining with uranyl acetate and alkaline lead citrate (each 5 to 10 min) and analysis under a Hitachi Model H-7650 TEM (HITACHI, Tokyo, Japan).
For SEM, the specimens were fixed and post-fixed as described above for TEM. Then, they were transferred into ethanol:iso-amyl acetate (1:1 v/v) for 30 min, and iso-amyl acetate overnight. After dehydration in a Hitachi Model HCP-2 critical point dryer (HITACHI, Tokyo, Japan) with liquid CO₂, the specimens were coated with gold-palladium on a Hitachi Model E-1010 ion sputter (4–5 min) before analysis on a Hitachi Model TM-1000 SEM.

Xiao C.W., Liu Y., Wei Q., Ji Q.A., Li K., Pan L.J, & Bao G.L. (2019). Inhibitory Effects of Berberine Hydrochloride on Trichophyton mentagrophytes and the Underlying Mechanisms. Molecules, 24(4), 742.

+ Open protocol

+ Expand

Petal Epidermal Cell and Vacuole Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

According to previous studies [25 , 48 (link)], SEM and TEM were carried out and were observed and photographed in Hitachi Model TM-1000 SEM and Model H-7650 respectively in Zhejiang University. Cell size of adaxial and abaxial petal epidermal cells, and vacuole size were manually measured using Image J software (http://rsb.info.nih.gov/ij/, NIH, MD, USA).

Fu J., Zhang C., Liu Y., Pang T., Dong B., Gao X., Zhu Y, & Zhao H. (2020). Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans. BMC Plant Biology, 20, 337.

+ Open protocol

+ Expand

Leaf Ultrastructural Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The infected leaves were cut into small pieces and first fixed with 2.5% glutaraldehyde in phosphate buffer (PBS) (0.1 M, pH7.0) overnight. Then, samples were washed three times and post-fixed with 1% OsO4 in PBS for 2 h. After double fixation, the samples were dehydrated in a graded series of ethanol (30, 50, 70, 80, 90, and 100%) for 20 min at each step and then transferred to pure isoamyl acetate (v:v = 1:1) overnight. Finally, the samples were dehydrated in a Hitachi Model HCP-2 critical point dryer with liquid CO₂. The dehydrated samples were coated with gold-palladium in a Hitachi Model E-1010 ion sputter for 5 min and observed in a Hitachi Model TM-1000 SEM.

Liu N., Xu S., Yao X., Zhang G., Mao W., Hu Q., Feng Z, & Gong Y. (2016). Studies on the Control of Ascochyta Blight in Field Peas (Pisum sativum L.) Caused by Ascochyta pinodes in Zhejiang Province, China. Frontiers in Microbiology, 7, 481.

+ Open protocol

+ Expand

Transcriptome Analysis of Osmanthus fragrans

Check if the same lab product or an alternative is used in the 5 most similar protocols

According to previous studies [49, 50] , SEM and TEM were carried out and were observed and photographed in Hitachi Model TM-1000 SEM and Model H-7650 respectively in Zhejiang University. Cell size of adaxial and abaxial petal epidermal cells, and vacuole size were manually measured using Image J software (http://rsb.info.nih.gov/ij/, NIH, MD, USA).
RNA extraction, cDNA library preparation and sequencing RNA isolation and RNA puri cation of 18 samples were carried out as described previously [51] . The cDNA library preparation and sequencing of 18 samples of O. fragrans were respectively performed as described previously [52] for RNA-seq analysis. What's more, a RNA pool mixed from 18 RNA samples was used to construct a cDNA library for reference transcriptome sequencing. The Illumina sequencing was performed at the Beijing Genomics Institute (BGI) (Shenzhen, China) (http://www.genomics.cn/index.php) according to the manufacturer's instructions (Illumina Inc, San Diego, CA, USA). A total of 18 sets of raw reads were obtained for RNA-seq analysis, corresponding to H2-1, H2-2, H2-3, H4-1, H4-2, H4-3, H6-1, H6-2, H6-3, L2-1, L2-2, L2-3, L4-1, L4-2, L4-3, L6-1, L6-2, and L6-3, while raw reads were obtained for reference transcriptome corresponding to ALL.

Fu J., Zhang C., Liu Y., Pang T., Dong B., Gao X., Zhu Y., & Zhao H. (2020). Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!