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Magnapure isolation kit and instrument

Manufactured by Roche
Sourced in Germany

The Magnapure Isolation Kit is a nucleic acid extraction system designed for automated high-throughput purification of DNA and RNA from various sample types. The accompanying Magnapure instrument automates the extraction process, providing consistent and reliable results. The core function of this product is to efficiently isolate and purify nucleic acids from samples for downstream analytical applications.

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3 protocols using magnapure isolation kit and instrument

1

PBMC Isolation and RNA Extraction

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In the STORK study, PBMC was isolated from venous blood using BD Vacutainer CPT Tubes (BD, NJ) at weeks 22–24, 36–38, and at 5-year follow-up and stored at − 70 °C until extraction. RNA was extracted using Magnapure Isolation Kit and instrument (Roche Life Science, Penzberg, Germany) at weeks 22–24 and with Magmax isolation kit and instrument (Applied Biosystems, Carlsbad, CA) at weeks 36–38 and at follow-up, due to change in instruments at the laboratory over the years, as previously published19 . RNA available from PBMC in this cohort included 189 controls/32 PE at 22–24 weeks, 194 controls/24 PE at 36–38 weeks and 213 controls/10 PE at 5-year follow-up.
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2

Telomere Length Quantification from PBMC

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DNA was extracted from the PBMC isolated from venous blood at weeks 22–24 (controls n = 186, PE n = 31) using Magnapure Isolation Kit and instrument (Roche Life Science) and using a salting out procedure21 (link) of EDTA blood collected at the 5-year follow-up visit in the STORK study (controls n = 206, PE n = 10). Equal amounts of DNA (2 ng/μL) were used to measure telomere length by PCR using telomere‐specific primers relatively quantified to the single‐copy gene RPLP0 (Table 1, Supplemental File). All samples were run in duplicates. Quantification was performed using SYBR Green PCR Fast Mix (Quantabio, Beverly, MA) on an ABI Prism 7900 (Applied Biosystems).
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3

PBMC Isolation and RNA Extraction

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PBMC was isolated from venous blood using BD Vacutainer CPT Tubes (BD, NJ, USA) at weeks 22-24, 36-38 and at follow-up and stored at -70°C until extraction. RNA was extracted using MagNA Pure Isolation Kit and Instrument (Roche Life Science, Penzberg, Germany) at weeks 22-24 and with MagMAX Isolation Kit and Instrument (Applied Biosystems, Carlsbad, CA, USA) at weeks 36-38 and at follow-up, due to change in instruments at the laboratory over the years.
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