Western blotting analysis was performed as usually reported [34 (link)]. The GIST-T1 and GIST-T1/IMR cells were challenged with rabbit polyclonal anti-S1P (ab59870), anti-SPK1 (ab260073), anti-β-actin antibodies (ab8826) (Abcam company) and ABCC1 (DF7148) (Affinity company). Proteins were visualized with enhanced chemiluminescence detection reagent according to the manufacturer’s instructions (Pierce, Rockford, Illinois).
Df7148
Manufactured by Affinity Biosciences
The DF7148 is a laboratory instrument designed for liquid handling and sample preparation. Its core function is to accurately and precisely dispense and aspirate various liquid samples and reagents.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using df7148
1
Western Blot Analysis of GIST-T1 Cells
2
Rat Hippocampus Protein Expression Analysis
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Rat hippocampus was lysated in RIPA Lysis Buffer (Beyotime) containing (Biyuantian, Wuhan, China). Protein concentrations of each sample were measured by BCA assay. Equal protein amounts were subjected to SDS‐PAGE and transferred onto PVDF membranes. After incubation with the primary antibodies—anti‐GAPDH (1:10000, 2118S; Cell Signaling Technology), anti‐LRP4 (1:2000, DF9610; Affinity), anti‐ABCC1 (1:1000, DF7148; Affinity), anti‐MDM4 (1:1000, DF7532; Affinity), anti‐p53 (1:2000, AF0879; Affinity), anti‐TMBIM1 (1:1000, DF4580; Affinity), anti‐PSD‐95 (6G6‐1C9, 1:250; Abcam), and anti‐SV2A (1:100; DSHB) overnight at 4°C—the membranes were incubated with second antibodies (goat anti‐rabbit IgG, HRP‐conjugate, 1:5000, BL003A; Biosharp) at room temperature for 2 h. Chemiluminescent values of the protein of interest were divided by its corresponding GAPDH chemiluminescent values.
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