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Ccr6ax488 clone tg7 ccr6

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CCR6Ax488 (clone TG7/CCR6) is a fluorescently labeled monoclonal antibody that binds to the CCR6 receptor. CCR6 is a chemokine receptor expressed on various immune cell types.

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Lab products found in correlation

Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm permeabilization solution, by BD (2 mentions) Cd4apc clone rpa t4, by BD (2 mentions) Ccr10pe clone6588 5, by BioLegend (2 mentions) Tcr vβ12, by Thermo Fisher Scientific (2 mentions) Tcr vβ14, by Thermo Fisher Scientific (2 mentions) Tcr vβ1, by Thermo Fisher Scientific (2 mentions) Tcr vβ2, by Thermo Fisher Scientific (2 mentions) Tcr vβ7, by Thermo Fisher Scientific (2 mentions) Tcr vβ13, by Thermo Fisher Scientific (2 mentions) Tcr vβ abs, by Beckman Coulter (2 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions)

2 protocols using ccr6ax488 clone tg7 ccr6

1

Comprehensive T Cell Immunophenotyping Protocol

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The reagent panels used in the present study are listed in Supplementary Table 1. All except the
“Th subset” and “sorting” panels have been described in
previous publications [17 (link), 18 (link)]. The “Th subset” panel included the following
additional reagents: CCR6Ax488 (clone TG7/CCR6); CCR10PE (clone
6588-5, both from BioLegend); CXCR3PE-Cy5 (clone 1C6/CXCR3); and
HLA-DRPE-Cy5.5 (clone TÜ36, both from BD Biosciences). The
“sorting” panel included the following additional reagents:
CCR6BV605 (clone G034E3); CCR4PE-Cy7 (clone TG6/CCR4, both from
BioLegend); CD4APC (clone RPA-T4, BD Biosciences); as well as TCR-Vβ12
(clone VER2.32.1); TCR-Vβ14 (clone CAS1.1.1.3); and TCR-Vβ17 (clone
E17.5F3.15.13) conjugated to FITC (Life Technologies) at the VRC; and TCR-Vβ1
(clone BL37.2); TCR-Vβ2 (clone MPD2D5); TCR-Vβ7 (clone ZOE);
TCR-Vβ13.6 (clone JU74.3); TCR-Vβ16 (clone TAMAYA1.2); and
TCR-Vβ22 (clone IMMU546) conjugated to Ax594 (Life Technologies) at the VRC. All
unconjugated TCR-Vβ Abs were obtained from Beckman Coulter. For intracellular
staining, cells were treated with BD Cytofix/Cytoperm Permeabilization Solution (BD
Biosciences), except for the Treg panel, where the Foxp3 Staining Buffer Set
was employed (eBioscience). Data were acquired on an LSR II (BD Biosciences) using a
high-throughput system (HTS).
Mahnke Y.D., Fletez-Brant K., Sereti I, & Roederer M. (2016). Reconstitution of Peripheral T Cells by Tissue-Derived CCR4+ Central Memory Cells Following HIV-1 Antiretroviral Therapy. Pathogens & Immunity, 1(2), 260-290.
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2

Multiparameter Flow Cytometry Panels

Check if the same lab product or an alternative is used in the 5 most similar protocols
The reagent panels used in the present study are listed in Supplementary Table 1. All except the “Th subset” and “sorting” panels have been described in previous publications [17 (link), 18 (link)]. The “Th subset” panel included the following additional reagents: CCR6Ax488 (clone TG7/ CCR6); CCR10PE (clone 6588-5, both from BioLegend); CXCR3PE-Cy5 (clone 1C6/CXCR3); and HLA-DRPE-Cy5.5 (clone TÜ36, both from BD Biosciences). The “sorting” panel included the following additional reagents: CCR6BV605 (clone G034E3); CCR4PE-Cy7 (clone TG6/CCR4, both from BioLegend); CD4APC (clone RPA-T4, BD Biosciences); as well as TCR-Vβ12 (clone VER2.32.1); TCR-Vβ14 (clone CAS1.1.1.3); and TCR-Vβ17 (clone E17.5F3.15.13) conjugated to FITC (Life Technologies) at the VRC; and TCR-Vβ1 (clone BL37.2); TCR-Vβ2 (clone MPD2D5); TCRVβ7 (clone ZOE); TCR-Vβ13.6 (clone JU74.3); TCR-Vβ16 (clone TAMAYA1.2); and TCR-Vβ22 (clone IMMU546) conjugated to Ax594 (Life Technologies) at the VRC. All unconjugated TCRVβ Abs were obtained from Beckman Coulter. For intracellular staining, cells were treated with BD Cytofix/Cytoperm Permeabilization Solution (BD Biosciences), except for the Treg panel, where the Foxp3 Staining Buffer Set was employed (eBioscience). Data were acquired on an LSR II (BD Biosciences) using a high-throughput system (HTS).
Mahnke Y.D., Fletez-Brant K., Sereti I, & Roederer M. (2016). Reconstitution of Peripheral T Cells by Tissue-Derived CCR4+ Central Memory Cells Following HIV-1 Antiretroviral Therapy. Pathogens & Immunity, 1(2), 260-290.
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