An emulsion-solvent evaporation technique was developed to synthesize the NPs having varying densities of curdlan (2, 5 and 8% w/w).15 (link)–18 Briefly, 2 mL of a 1% w/v polymer solution (mixture of C-PLGA and PLGA) in a DMSO: DCM mixture (1:9) was added drop-wise to 10 mL of a PVA solution in de-ionised water (0.5% w/v) under probe sonication (Bandelin Sonoplus UW2070 + HD 2070 Berlin, Germany) for 7 min, 55% power, 70 Watt). A mixture of C-PLGA and PLGA was used to produce the desired concentration of curdlan functionalized NPs. For example, to synthesize 2% w/w curdlan loaded PLGA NPs, a ratio of 4 parts PLGA: 1-part C-PLGA was used. The same procedure was used to synthesize NPs containing 5% and 8% w/w curdlan loaded PLGA NPs. PLGA NPs were synthesized using only a solution of PLGA polymer. The solvent was subsequently removed in vacuo at 35°C and the NPs collected through centrifugation at 10 000 × g for 5 min, followed by washing once with de-ionized water and lyophilization over 72 h in the presence of sucrose (1% w/v) as a cryoprotectant.
Sonoplus uw2070 hd 2070
Manufactured by Bandelin
Sourced in Germany
The Sonoplus UW2070 + HD 2070 is a laboratory equipment product. It is designed for ultrasonic homogenization and dispersion of samples. The device features a stable and powerful ultrasonic generator.
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2 protocols using sonoplus uw2070 hd 2070
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Curdlan-Loaded PLGA Nanoparticle Synthesis
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Curdlan-Loaded PLGA Nanoparticle Synthesis
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An emulsion-solvent evaporation technique was developed to synthesize the NPs having varying densities of curdlan (2, 5 and 8% w/w).15 (link)–18 Briefly, 2 mL of a 1% w/v polymer solution (mixture of C-PLGA and PLGA) in a DMSO: DCM mixture (1:9) was added drop-wise to 10 mL of a PVA solution in de-ionised water (0.5% w/v) under probe sonication (Bandelin Sonoplus UW2070 + HD 2070 Berlin, Germany) for 7 min, 55% power, 70 Watt). A mixture of C-PLGA and PLGA was used to produce the desired concentration of curdlan functionalized NPs. For example, to synthesize 2% w/w curdlan loaded PLGA NPs, a ratio of 4 parts PLGA: 1-part C-PLGA was used. The same procedure was used to synthesize NPs containing 5% and 8% w/w curdlan loaded PLGA NPs. PLGA NPs were synthesized using only a solution of PLGA polymer. The solvent was subsequently removed in vacuo at 35°C and the NPs collected through centrifugation at 10 000 × g for 5 min, followed by washing once with de-ionized water and lyophilization over 72 h in the presence of sucrose (1% w/v) as a cryoprotectant.
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