pcDNA3.1-LINC01116, sh-LINC01116, sh-TPM1, pcDNA3.1-EZH2, sh-EZH2 and their negative controls were acquired from GenePharma (Shanghai, China). Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA) was used for transfection following the manufacturer’s protocol.
Sh tpm1
Manufactured by GenePharma
Sourced in China
The Sh-TPM1 is a laboratory instrument designed for the extraction and purification of RNA from various biological samples. It utilizes a unique thermal pressure-based method to efficiently isolate high-quality RNA with minimal sample loss. The core function of the Sh-TPM1 is to provide a reliable and reproducible method for RNA extraction, which is a critical step in numerous molecular biology and genomic research applications.
Automatically generated - may contain errors
Lab products found in correlation
Shezh2, by GenePharma (1 mentions)
Pcdna3.1 linc01116, by GenePharma (1 mentions)
Pcdna3.1 ezh2, by GenePharma (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 vector, by GenePharma (1 mentions)
Oe neat1, by GenePharma (1 mentions)
Sh neat1, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
2 protocols using sh tpm1
1
Transfection of LINC01116 and EZH2 Plasmids
2
Manipulating NEAT1 and TPM1 in fBMFs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Synthesized short hairpin RNA and overexpression vector for NEAT1 (sh-NEAT1 or oe-NEAT1), shRNA targeting TPM1 (sh-TPM1), and their negative control (NC) were synthesized and obtained from Genepharma (Shanghai, China). The miR-760 mimics, miR-760 inhibitor, mimics NC and inhibitor NC were also synthesized and bought from Genepharma. For the overexpressing of TPM1, the full-length human TPM1 cDNA was synthesized and sub-cloned into the pcDNA3.1 vector (Genepharma). Transient transfection of fBMFs (single or cotreated transfection) were performed by the Lipofectamine 2000 which obtained from Invitrogen (Carlsbad, CA, USA, #11668-027) in according with the manufacturer's protocol.
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