Potassium chloride (kcl)

Manufactured by MP Biomedicals

Sourced in United States

Potassium chloride (KCl) is a chemical compound that is commonly used as a laboratory reagent. It is a colorless, crystalline solid that is highly soluble in water. KCl is an ionic compound composed of potassium and chloride ions. Its core function is to provide a source of potassium ions for various experimental and analytical applications.

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Lab products found in correlation

Sodium chloride (nacl), by MP Biomedicals (6 mentions) Ammonium acetate, by MP Biomedicals (4 mentions) Na2hpo4, by MP Biomedicals (4 mentions) Mgcl2, by MP Biomedicals (4 mentions) Ampicillin, by MP Biomedicals (4 mentions) Kh2po4, by MP Biomedicals (4 mentions) Nh4cl, by Merck Group (4 mentions) Cacl 2 2h 2 0, by Merck Group (4 mentions) Sodium chloride (nacl), by Avantor (4 mentions) Hydrochloric acid (hcl), by Avantor (4 mentions) Ethanol, by Avantor (4 mentions) Mgcl2, by Merck Group (4 mentions) Urea, by Avantor (4 mentions) Ethylene glycol tetraacetic acid (egta), by MP Biomedicals (3 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by MP Biomedicals (3 mentions) Pensterp cocktail, by Thermo Fisher Scientific (3 mentions) Advanced dmem f12 media, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Protease inhibitor cocktail, by Roche (3 mentions) Diethyl ether, by Honeywell (3 mentions)

11 protocols using potassium chloride (kcl)

Western Blot with Protein Extraction

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RbCl was obtained from Chem-Impex (#07221, Illinois, Wood Dale, IL, USA). TCA (#BP555), ECL kit (#34096), Trizol reagent (#15596026), 100X Halt™ phosphatase inhibitor Cocktail (#78420), RIPA buffer (#89900) were from Thermofisher Scientific (Massachusetts, Waltham, MA, USA). NaCl (#194848) and KCl (#194844) was obtained from MP Biomedicals (Ohio, Solon, OH, USA). Mannitol was from AppliChem (#142067.1210, Darmstadt, Germany). HEPES was obtained from Flow Laboratories (#15-884-15, California, Hyland Ave Costa Mesa, CA, USA). MgCl₂ was purchased from Honeywell (#63020, Charlotte, North Carolina, USA). Nonidet P-40 was obtained from Helicon (Am-E109, Moscow, Russia). Secondary anti-rabbit HRP-conjugated antibodies (#AP132P) were obtained from Millipore (California, Temecula, CA, USA). Quick-RNA MicroPrep microkit columns was obtained from Zymo Research (#R1051, California, Irvine, CA, USA). The ImProm-IITM Reverse Transcription System kit was obtained from Promega, (#A3800, Wisconsin, Madison, WI, USA). QIAquick Gel Extraction Kit was obtained from Qiagen (#28704, Maryland, Germantown, MD, USA). Unless otherwise noted, all chemicals were of the purest grade.

Fedorov D.A., Sidorenko S.V., Yusipovich A.I., Parshina E.Y., Tverskoi A.M., Abramicheva P.A., Maksimov G.V., Orlov S.N., Lopina O.D, & Klimanova E.A. (2021). Na+i/K+i imbalance contributes to gene expression in endothelial cells exposed to elevated NaCl. Heliyon, 7(9), e08088.

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Molecular Techniques for Protein Analysis

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Hepes, Tris, KCl, NaCl, sodium phosphate, synthetic defined medium -Leu-Trp-His (SD medium), 3-amino-1,2,4-triazole (3-AT), and IPTG were purchased from MP Biomedicals, and LB broth and LB agar were obtained from HiMedia. Anti-flagellin and anti-GroEL antibodies were purchased from Abcam. The remaining chemicals used were of molecular biology grade and were obtained from Sigma-Aldrich.

Pradhan P., Taviti A.C, & Beuria T.K. (2024). The bacterial division protein MinDE has an independent function in flagellation. The Journal of Biological Chemistry, 300(4), 107117.

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Biochemical Assay for MANT-ATP

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HEPES, KCl, and sodium phosphate were from MP Biomedicals; LB broth and LB agar were from HiMedia, and MANT-ATP was from Invitrogen. All other chemicals used were of molecular biology grade and purchased from Sigma–Aldrich.

Mallick S., Kumar A., Dodia H., Alexander C., Vasudevan D, & Beuria T.K. (2021). Biochemical characterization of an E. coli cell division factor FtsE shows ATPase cycles similar to the NBDs of ABC-transporters. Bioscience Reports, 41(1), BSR20203034.

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Limonoid Extraction and Characterization

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MES, heparin, BES, BSA, BCA, CuSO₄, ThS and ANS were purchased from Sigma. IPTG and DTT were purchased from Calbiochem. Other chemicals such as ampicillin, NaCl, KCl, Na₂HPO₄, KH₂PO₄, EGTA, MgCl₂, PMSF, ammonium acetate were from MP and protease inhibitor cocktail was from Rosche. Dulbecco modified Eagle’s media (DMEM), Fetal Bovine Serum (FBS), Penicillin-Streptomycin were purchased from Gibco (Thermo fisher Scientific), and MTT (Thiazolyl blue tetrazolium bromide) was purchased from Sigma. The basic limonoids used here were isolated from fruit of A. indica. These compounds were characterized and well matched with that of reported data³⁵.

Gorantla N.V., Das R., Chidambaram H., Dubey T., Mulani F.A., Thulasiram H.V, & Chinnathambi S. (2020). Basic Limonoid modulates Chaperone-mediated Proteostasis and dissolve Tau fibrils. Scientific Reports, 10, 4023.

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Biochemical Assays and Cell Culture Reagents

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MES (M3671), BSA (9048-46-8), BES (10191-18-1), BCA (B9643), CuSO4 (C2284), ThS (T1892), MTT (M2128) and Rose Bengal (330000) were purchased from Sigma. IPTG (420322) and DTT (3870) were purchased from Calbiochem. Other chemicals such as Ampicillin (2007081), NaCl (194848), KCl (194844), Na₂HPO₄ (191437), KH₂PO₄ (19142), EGTA (194823), MgCl₂ (191421), PMSF (195381), Ammonium acetate (191404), Heparin (904108) and DMSO were from purchased from MP biomedicals and protease inhibitor cocktail was from Roche (11697498001). Copper coated carbon grids were purchased from Ted Pella (01814.F, carbon type-B, 400 mesh Cu), DMEM advanced F12 media (12634010), Fetal Bovine Serum (16000044), Pensterp cocktail (04693159001) and Anti-anti (15240062) were purchased from Gibco. Tubulin (Thermo PA1-41331), actin (Thermo MA5-15739) were procured from thermo, total Tau antibody K9JA was purchased from Dako (K9JA, Dako A0024). The secondary antibodies Alexa Fluor 488 (A11034) and Alexa Fluor 555 (A32727) were purchased from thermo.

Dubey T., Gorantla N.V., Chandrashekara K.T, & Chinnathambi S. (2020). Photodynamic exposure of Rose-Bengal inhibits Tau aggregation and modulates cytoskeletal network in neuronal cells. Scientific Reports, 10, 12380.

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Comprehensive Reagent Preparation for Biomolecular Research

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MES, heparin, BES, bicinchoninic
acid (BCA), CuSO₄, thioflavin S (ThS), ANS, toluidine blue,
MTT, and dimethyl sulfoxide (DMSO) were purchased from Sigma. IPTG
and dithiothreitol (DTT) were purchased from Calbiochem. Other chemicals
such as ampicillin, NaCl, KCl, Na₂HPO₄, KH₂PO₄, ethylene glycol tetraacetic acid (EGTA), MgCl₂, phenylmethylsulfonyl fluoride (PMSF), ammonium acetate,
and bovine serum albumin (BSA) were obtained from MP and protease
inhibitor cocktail was obtained from Roche. Copper-coated carbon grids
were purchased from Ted Pella, Inc. Advanced DMEM/F-12 media, fetal
bovine serum (FBS), pensterp cocktail, and anti-anti were purchased
from Gibco. All laboratory reagents used for Drosophila studies were purchased from Merck.

Dubey T., Gorantla N.V., Chandrashekara K.T, & Chinnathambi S. (2019). Photoexcited Toluidine Blue Inhibits Tau Aggregation in Alzheimer’s Disease. ACS Omega, 4(20), 18793-18802.

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Detailed Biomolecular Reagents Protocol

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MES (M3671), BSA (82516), BES (14853), BCA (B9643), CuSO 4 (C2284) ThS (T1892), Toluidine Blue (T3260), MTT (M2128) were purchased from Sigma. IPTG (420322) and DTT (3870) were purchased from Calbiochem. Other chemicals such as Ampicillin (2007081), NaCl (194848), KCl (194844) , Na 2 HPO 4 (191437), KH 2 PO 4 (19142) , EGTA (194823) , MgCl 2 (191421) , PMSF (195381) , Ammonium acetate (191404) and Heparin (904108), DMSO were from purchased from MP Biomedicals and protease inhibitor cocktail was from Roche. Copper coated carbon grids were purchased from Ted Pella (01814F, carbon type-B, 400 mesh, Cu) DMEM advanced F12 media (12634010), Fetal Bovine serum (16000044), Pensterp cocktail (04693159001), Anti-anti (15240062) were purchased from Gibco.

Dubey T., Gorantla N.V., & Chinnathambi S. (2020). Photo-excited Toluidine Blue disaggregates the Repeat Tau and modulates cytoskeletal structure in neuronal cells.

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Carrot-Based Pectin Extraction and Characterization

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Carrots (Daucus carota cv. Nerac) were purchased in a local shop and stored at 4°C. High methylesterified citrus pectin (CP) (Sigma Aldrich) was used as the starting material for the preparation of pectin with different degree of methyl-esterification (DM). Olive oil (extra virgin) was kindly donated by Vandemoortele (Ghent, Belgium). All chemicals and reagents were of analytical grade from Sigma Aldrich, except for NaCl, HCl, urea and ethanol (from VWR); CaCl 2 .2H 2 0, KClhCZIBPBHhf-iFeNIq/" target="_blank">NH 4 Cl, MgCl 2 and CaCl 2 .2H 2 0 (from Merck); hexane and acetone (from Chem Lab); glucose and NaHCO 3 (from Fisher Scientific); KCl (from MP Biomedicals).

Verrijssen T.A.J., Verkempinck S.H.E., Christiaens S., Van Loey A.M, & Hendrickx M.E. (2015). The effect of pectin on in vitro β-carotene bioaccessibility and lipid digestion in low fat emulsions. Food Hydrocolloids., 49.

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Carrot Bioactive Compound Extraction

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Fresh carrots (Daucus carota cv. Nerac) were purchased in a local shop in Belgium and stored at 4 °C.
Olive oil (extra virgin) was kindly donated by Vandemoortele (Ghent, Belgium). All chemicals and reagents were from Sigma Aldrich, except for NaCl, HCl, urea, anhydrous sodium sulphate and ethanol (from VWR); CaCl 2 .2H 2 0, KClhCZIBPBHhf-iFeNIq/" target="_blank">NH 4 Cl and MgCl 2 (from Merck); hexane, sulphuric acid and acetone (from Chem Lab); glucose and NaHCO 3 (from Fisher Scientific); heptane (from Fluka); KCl (from MP Biomedicals) and diethylether (from Riedel-De Haën). All chemicals and reagents were of analytical grade. The phosphatidylcholine Assay Kit is from Cayman Chemical Company.

Verrijssen T.A.J., Smeets K.H.G., Christiaens S., Palmers S., Van Loey A.M, & Hendrickx M.E. (2015). Relation between in vitro lipid digestion and β-carotene bioaccessibility in β-carotene-enriched emulsions with different concentrations of l-α-phosphatidylcholine. Food research international (Ottawa, Ont.), 67.

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Characterization of Lipid Ingredients

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Corn and olive oil were bought from a local supermarket and stored at room temperature in dark conditions until use. Cocoa butter was kindly donated by Barry Callebaut AG (Zurich, Switzerland), while fully hydrogenated coconut oil was donated by Vandemoortele (Gent, Belgium) and were kept at 4˚C in the dark until use. In the case of lipids with high melting temperature, the solid fat content (SFC) profiles in percentage of lipids in solid state (%) at different temperatures determined by nuclear magnetic resonance (NMR) is provided (supplementary material, Figure 1). The results for hydrogenated coconut oil were provided by the supplier while the results for cocoa butter were the ones reported by Geary & Hartel (2017) . Synthetic β-carotene was obtained from Sigma Aldrich (purity > 97.0 %). All other chemicals and reagents were obtained from Sigma Aldrich, except for NaCl, HCl, urea, anhydrous sodium sulphate and ethanol (from VWR); CaCl2.2H20, KClhCZIBPBHhf-iFeNIq/" target="_blank">NH4Cl and MgCl2 (from Merck); hexane, sulphuric acid and acetone (from Chem Lab); and NaHCO3 (from Fisher Scientific); heptane (from Fluka); KCl (from MP Biomedicals) and diethylether (from Riedel-De Haën). All chemicals and reagents were of analytical grade.

Salvia-Trujillo L., Verkempinck S., Rijal S.K., Van Loey A., Grauwet T, & Hendrickx M. (2019). Lipid nanoparticles with fats or oils containing β-carotene: Storage stability and in vitro digestibility kinetics. Food chemistry, 278.

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