Anti caspase 3 antibodies 9662

To study the induction of apoptosis, cells were treated with camptothecin (Wako, Osaka, Japan), doxorubicin (LC Laboratories, Woburn, MA), MG132 (Merck Millipore, Darmstadt, Germany), or exposed to γ-irradiation (Gammacell 40, Atomic Energy of Canada, Ontario, Canada). Expression of cleaved poly (ADP-ribose) polymerase (PARP) and cleaved caspase-3 was detected by western blot analysis using anti-cleaved PARP (9541) and anti-caspase-3 antibodies (9662), respectively (Cell Signaling Technology, Danvers, MA). Assessment of apoptosis was also performed by annexin V and PI double-staining using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Life Technologies). Briefly, cultured cells were treated with vehicle or camptothecin for 24 h. The cells were stained with Annexin V and PI, and subsequently analyzed on a FACSCalibur (Becton Dickinson, Franklin Lakes, NJ) using FlowJo software (Tree Star, Ashland, OR).

Chr-A was provided by Prof. Hua-Wei Zhang (Zhejiang University of Technology). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS, Cat# 164210-50) for cell cultures were purchased from Gibco BRL (Grand Island, NY, USA) and Procell (Wuhan, China), respectively. Anti-Bax antibodies (50599-2-Ig) and anti-Bcl-2 antibodies (12789-1-AP) were purchased from Proteintech (Manchester, United Kingdom). Anti-Caspase 3 antibodies (9662), anti-Cleaved Caspase 3 antibodies (9664), anti-Caspase 7 antibodies (12827), anti-Cleaved Caspase 7 antibodies (8438), anti-Caspase 9 antibodies (9508), anti-Cleaved Caspase 9 antibodies (20750), anti-Akt antibodies (9272), anti-p-Akt antibodies (9271), anti-GSK-3β antibodies (9315), anti-p-GSK-3β antibodies (9323), anti-slug antibodies (9585), anti-MMP2 antibodies (87809) and anti-GAPDH antibodies (5174) were purchased from Cell Signaling Technology (Beverley, CA, USA).