Asipro vm micro pet analysis software

PET images of KPL4-bearing BALB/c mice were obtained on an Inveon microPET scanner (Siemens, Knoxville, TN, USA). Tumor-bearing mice were injected with ¹⁸F-hyErbB2-idT aptamer (18.5–22.2MBq, 500 pmol) via the tail vein under 2% isoflurane anesthesia. Static PET scans were performed at 30 min and 1 h after injection with an acquisition time of 20 min. Images were reconstructed using a three-dimensional ordered subsets expectation maximization (3D-OSEM) algorithm. Image analysis was performed using the ASIPro VM^™ Micro PET Analysis software (Siemens Medical Solutions).

Three mice bearing both HER3/RH7777 and RH7777 tumors were injected with approximately 3.7 MBq of [⁸⁹Zr]Mab#58 (approximately 60 μg protein) via the tail vein. At 1, 2, 4, and 6 days post-injection, PET data acquisition was conducted for 10–20 min by using a small-animal PET system (Inveon, Siemens Medical Solutions, Malvern, PA) under isoflurane anesthesia during the entire scanning period. Images were reconstructed using a 3D maximum a posteriori (MAP) method (18 iterations with 16 subsets, β = 0.2) without attenuation correction. Image analysis was performed using the ASIPro VM Micro PET Analysis software (Siemens, Knoxville, TN). A lamp and heating pad were used during the PET scan to maintain the body temperature of the mice. Tracer uptake was expressed as % ID/g. The region of interest was manually drawn over tumors and tracer uptake was quantified using ASI Pro software (Siemens Medical Solutions). No correction for partial volume effects was performed.
Three mice bearing the CTOS C45 xenograft were injected with 3.7 MBq of [⁸⁹Zr]Mab#58 or ⁸⁹Zr labeled control antibody ([⁸⁹Zr]IgG), and PET data were acquired as mentioned above.