Total RNA was extracted from cells using TRIzol® Reagent (Life Technologies, Cat. No. 15596018), then cDNA was synthesized using ReverTra Ace® qPCR RT Master Mix (TOYOBO CO., LTD, Cat. No. FSQ-201). Quantitative real-time PCR was performed using Power Up™ SYBR™ Green Master Mix (Applied Biosystems, Cat. No. A25742) or THUNDERBIRD® Probe qPCR Mix (TOYOBO CO., LTD, Cat. No. QPS-101). As another procedure, using TaqMan™ Fast Advanced Cells-to-CT™ Kit (Invitrogen) in accordance with the manufacturer’s protocol, after cells were lysed cDNA was synthesized and then quantitative real-time PCR was performed. The TaqMan™ IDs (Applied Biosystems) of the gene expression assay were as follows: Gapdh (Mm99999915_g1), Alp (Mm00475834_m1), and Osteocalcin (Mm03413826_mH). Following are the sequences of the specific PCR primers (Life Technologies): Gapdh: 5ʹ-AAATGGTGAAGGTCGGTGG-3ʹ and 5ʹ-TGAAGGGGTCGTTGATGG-3ʹ, Npnt: 5ʹ-CACGAGTAATTACGGTTGACAACAG-3ʹ and 5ʹ-CTGCCGTGGAATGAACACAT-3ʹ.
Taqman ids
Manufactured by Thermo Fisher Scientific
The TaqMan™ IDs are molecular biology tools used for the detection and quantification of specific DNA or RNA sequences. They are based on the TaqMan™ technology, which utilizes fluorescent probes to monitor the amplification of target sequences during real-time PCR reactions.
Automatically generated - may contain errors
Lab products found in correlation
Taqman fast advanced cells to ct kit, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions)
Thunderbird probe qpcr mix, by Toyobo (2 mentions)
Revertra ace qpcr rt master mix, by Toyobo (2 mentions)
Specific pcr primers, by Thermo Fisher Scientific (1 mentions)
2 protocols using taqman ids
1
Total RNA Extraction and qPCR Analysis
2
Comprehensive RNA Extraction and qPCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from cells using TRIzol® Reagent (Life Technologies, Cat. No. 15596018), then cDNA was synthesized using ReverTra Ace® qPCR RT Master Mix (TOYOBO CO., LTD, Cat. No. FSQ-201).
Quantitative real-time PCR was performed using Power Up™ SYBR™ Green Master Mix (Applied Biosystems, Cat. No. A25742) or THUNDERBIRD® Probe qPCR Mix (TOYOBO CO., LTD, Cat. No. QPS-101). As another procedure, using TaqMan™ Fast Advanced Cells-to-CT™ Kit (Invitrogen) in accordance with the manufacturer's protocol, after cells were lysed cDNA was synthesized and then quantitative realtime PCR was performed. The TaqMan™ IDs (Applied Biosystems) of the gene expression assay were as follows: Gapdh (Mm99999915_g1), Alp (Mm00475834_m1), and Osteocalcin (Mm03413826_mH). Following are the sequences of the speci c PCR primers (Life Technologies): Gapdh: 5'-AAATGGTGAAGGTCGGTGG-3' and 5'-TGAAGGGGTCGTTGATGG-3', Npnt: 5'-CACGAGTAATTACGGTTGACAACAG - 3' and 5'-CTGCCGTGGAATGAACACAT-3'.
Quantitative real-time PCR was performed using Power Up™ SYBR™ Green Master Mix (Applied Biosystems, Cat. No. A25742) or THUNDERBIRD® Probe qPCR Mix (TOYOBO CO., LTD, Cat. No. QPS-101). As another procedure, using TaqMan™ Fast Advanced Cells-to-CT™ Kit (Invitrogen) in accordance with the manufacturer's protocol, after cells were lysed cDNA was synthesized and then quantitative realtime PCR was performed. The TaqMan™ IDs (Applied Biosystems) of the gene expression assay were as follows: Gapdh (Mm99999915_g1), Alp (Mm00475834_m1), and Osteocalcin (Mm03413826_mH). Following are the sequences of the speci c PCR primers (Life Technologies): Gapdh: 5'-AAATGGTGAAGGTCGGTGG-3' and 5'-TGAAGGGGTCGTTGATGG-3', Npnt: 5'-CACGAGTAATTACGGTTGACAACAG - 3' and 5'-CTGCCGTGGAATGAACACAT-3'.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!