Methanol (99.9%), formic acid, sodium hydroxide, sodium dihydrogen phosphate dehydrate, sodium carbonate and calcium chloride dehydrate (CaCl2(H2O)2) were purchased from Merck (Darmstadt, Germany). Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) reagent, 2,2-Azino-bis-3-etilbenzotiazolin-6-sulfonic acid (ABTS) reagent, Trolox, hydrochloric acid, p-coumaric acid, trans-ferulic acid, caffeic acid, p-hydroxybenzoic acid, gallocatechin, 4-vinylphenol, 4-vinylguaiacol, α-amylase (EC 232-565-6) from porcine pancreas (enzyme activity 5 U/mg solid), pepsin (EC 232-629-3) from porcine gastric mucosa (enzyme activity ≥ 2500 U/mg protein) and pancreatin (EC 232-468-9) from porcine pancreas (4 × USP specifications) were purchased from Sigma-Aldrich (Steinheim, Germany). Bile bovine (EC 232-369-0) was from Millipore (ZDA). Potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), sodium bicarbonate (NaHCO3), sodium chloride (NaCl), and magnesium chloride hexahydrate (MgCl2(H2O)6) were purchased from Sigma-Aldrich (Steinheim, Germany). Ammonium carbonate ((NH4)2CO3) was from Honeywell/Riedel-de Haen (Seelze, Germany). All chemicals and reagents used were of analytical quality. For preparation of working solutions ultrapure water (Milli-Q; Millipore, Bedford, MA, USA) was used.
4 vinylphenol
Manufactured by Merck Group
Sourced in Germany
4-vinylphenol is a chemical compound used as a laboratory reagent. It is a colorless, crystalline solid with the molecular formula C8H8O. 4-vinylphenol is used in various chemical and biochemical applications, including as an intermediate in organic synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Caffeic acid, by Merck Group (3 mentions)
P coumaric acid, by Merck Group (3 mentions)
2 methoxy 4 vinylphenol, by Merck Group (3 mentions)
Methanol, by Merck Group (2 mentions)
4 vinylguaiacol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Vanillin, by Merck Group (2 mentions)
Phenylacetaldehyde, by Merck Group (2 mentions)
Acetaldehyde, by Merck Group (2 mentions)
2 phenylethanol, by Merck Group (2 mentions)
3 methylbutanoic acid, by Merck Group (2 mentions)
2 3 butanedione, by Merck Group (2 mentions)
3 methyl 1 butanol, by Merck Group (2 mentions)
Methylpropanal, by Merck Group (2 mentions)
3 hydroxy 4 5 dimethyl 2 5h furanone, by Merck Group (2 mentions)
2 methoxy 4 methylphenol, by Merck Group (2 mentions)
2 phenylacetic acid, by Merck Group (2 mentions)
Dimethyl sulfide, by Merck Group (2 mentions)
Butanoic acid, by Merck Group (2 mentions)
2 methylbutanal, by Merck Group (2 mentions)
6 protocols using 4 vinylphenol
1
In Vitro Antioxidant Analysis
2
Quantification of Phenolic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethyl gallate, 4-hydroxybenzaldehyde, vanillin, tyrosol, protocatechuic aldehyde, syringaldehyde, pyrogallol, 4-hydroxy-3-methoxycinnamaldehyde, 4-methylcatechol, tryptophol, ethyl 3,4-dihydroxycinnamate, 2,6-dimethoxyphenol, ethyl vanillate, homovanillyl alcohol, eugenol, 4-hydroxy-3-methoxyphenylacetone, salicylaldehyde, coniferyl alcohol, epicatechin, catechin, scopoletin, p-coumaric acid, caffeic acid, resveratrol, syringic acid, rutin, gallic acid, salicylic acid, protocatechuic acid, 2-methoxy-4-vinylphenol, 4-vinylphenol, 4-ethylguaiacol were purchased from Sigma-Aldrich (Steinheim, Germany). All chemicals were used in the highest purity available. Acetonitrile and methanol (gradient grade for liquid chromatography) were also purchased from Sigma-Aldrich. Deionized water was produced by Mili-Q® Reference System (Merck KGaA, Darmstadt, Germany). Sodium chloride was purchased by Lach-Ner (Neratovice, Czech Republic). Standard solution of n-alkanes C8-C33 was purchased by CPAchem (Stara Zagora, Bulgaria). Technical gases, i.e., helium 4.6, hydrogen, and nitrogen, were purchased from Linde Gas (Prague, Czech Republic).
3
Microbial Conversion of Hydroxycinnamic Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Luria–Bertani (LB) medium was used for cell cultivation of E. coli. Hydroxycinnamic acids and the corresponding 4-vinylphenol derivatives, including FA, p-coumaric acid, caffeic acid, 4-vinylguaiacol, and 4-vinylphenol, were purchased from Sigma–Aldrich (St. Louis, MO, United States). Isopropyl-β-D thiogalactopyranoside (IPTG) was also obtained from Sigma–Aldrich. Yeast extract and tryptone were purchased from Oxoid (Basingstoke, England). Kanamycin was provided by Amresco (Solon, OH, United States). All the organic solvents used for whole-cell catalysis and high-performance liquid chromatography (HPLC) were of HPLC grade. The other chemicals used in this study were of analytical grade and were commercially available.
4
Flavor Compound Procurement for Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diethyl ether, dansyl chloride, and saturated
alkane standards were purchased from Sigma-Aldrich (Gillingham, Dorset,
UK). O-(2,3,4,5,6-Pentafluorobenzyl)-hydroxylamine
hydrochloride (PFBHA) was purchased from Fluka (Loughborough, UK).
The following food-grade aroma compounds were purchased from Sigma-Aldrich
(purity in parenthesis): acetaldehyde (≥99%), acetic acid (≥99.5%),
2,3-butanedione (97%), butanoic acid (≥99%), (E)-β-damascenone (≥98%), dimethyl sulfide (≥99%),
5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone (97%),
5(or 2)-ethyl-4-hydroxy-2(or 5)-methyl-3(2H)-furanone
(96%), (Z)-4-heptenal (≥98%), 3-hydroxy-4,5-dimethyl-2(5H)-furanone (10% in propylene glycol), 4-hydroxy-2,5-dimethyl-3(2H)-furanone (≥98%), methional (≥97%), 2-methoxy-4-methylphenol
(≥98%), 2-methoxyphenol (≥99%), 2-methoxy-4-vinylphenol
(≥98%), 2-methylbutanal (≥95%), 3-methylbutanal (≥97%),
3-methylbutanoic acid (99%), 3-methyl-1-butanol (≥98%), methylpropanal
(≥98%), 2,3-pentanedione (≥96%), phenylacetaldehyde
(10% in ethanol), 2-phenylacetic acid (≥99%), 2-phenylethanol
(≥99%), vanillin (≥97%), and 4-vinylphenol (10% in propylene
glycol). Encapsulated 3-methyl-2-butene-1-thiol flavor standard (0.02–4
ng/g) was purchased from FlavorActiV (Aston Rowant, UK).
alkane standards were purchased from Sigma-Aldrich (Gillingham, Dorset,
UK). O-(2,3,4,5,6-Pentafluorobenzyl)-hydroxylamine
hydrochloride (PFBHA) was purchased from Fluka (Loughborough, UK).
The following food-grade aroma compounds were purchased from Sigma-Aldrich
(purity in parenthesis): acetaldehyde (≥99%), acetic acid (≥99.5%),
2,3-butanedione (97%), butanoic acid (≥99%), (E)-β-damascenone (≥98%), dimethyl sulfide (≥99%),
5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone (97%),
5(or 2)-ethyl-4-hydroxy-2(or 5)-methyl-3(2H)-furanone
(96%), (Z)-4-heptenal (≥98%), 3-hydroxy-4,5-dimethyl-2(5H)-furanone (10% in propylene glycol), 4-hydroxy-2,5-dimethyl-3(2H)-furanone (≥98%), methional (≥97%), 2-methoxy-4-methylphenol
(≥98%), 2-methoxyphenol (≥99%), 2-methoxy-4-vinylphenol
(≥98%), 2-methylbutanal (≥95%), 3-methylbutanal (≥97%),
3-methylbutanoic acid (99%), 3-methyl-1-butanol (≥98%), methylpropanal
(≥98%), 2,3-pentanedione (≥96%), phenylacetaldehyde
(10% in ethanol), 2-phenylacetic acid (≥99%), 2-phenylethanol
(≥99%), vanillin (≥97%), and 4-vinylphenol (10% in propylene
glycol). Encapsulated 3-methyl-2-butene-1-thiol flavor standard (0.02–4
ng/g) was purchased from FlavorActiV (Aston Rowant, UK).
5
Synthesis and Characterization of Boron-Containing Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reactions were performed under an atmosphere of dinitrogen employing standard Schlenk techniques. Acetonitrile and dichloromethane were purchased from Merck and distilled from sodium benzophenone before use. Commercial grade acetone, hexane, ethyl acetate, tetrahydrofuran, and methanol were used without further purification. Compounds [Bu4N][B12H11(C4H8O2)] (2 ) [93 (link)] and [3,3′-Co(8-C4H8O2-1,2-C2B9H10)(1′,2′-C2B9H11)] (3 ) [94 (link)] and T8-COSAN [43 (link)] were synthesised according to the literature. Compounds [Bu4N]Br, Grubbs 1st generation catalyst [RuCl2(CHPh)-(PCy3)2], octavinylsilsesquioxane (OVS ), 1,3,5-tris(4-hydroxyphenyl)benzene (1 ) and 4-vinylphenol solution 10 wt% in propylene glycol were purchased from Sigma-Aldrich (Merck Life Science, Madrid, Spain). K2CO3 was obtained from Labkem (Barcelona, Spain).
6
Aroma Compound Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following aroma compounds were purchased from Sigma-Aldrich (purity in parenthesis): acetaldehyde (≥99 %), acetic acid (≥99.5 %), 2,3-butanedione (97 %), butanoic acid (≥99 %), dimethyl sulfide (≥99 %), 5(or 2)-ethyl-4-hydroxy-2(or 5)-methyl-3(2H)-furanone (homofuraneol, 96 %), Z-4-heptenal (≥98 %), 3-hydroxy-4,5-dimethyl-2(5H)-furanone (sotolone, 10 % in propylene glycol), 4-hydroxy-2,5-dimethyl-3(2H)-furanone (furaneol, ≥98 %), methional (≥97 %), 2'methoxyacetophenone (99 %), 2-methoxy-4-methylphenol (≥98 %), 2-methoxyphenol (≥99 %), 2methoxy-4-vinylphenol (≥98%), 2-methylbutanal (≥95 %), 3-methylbutanal (≥97 %), 3methylbutanoic acid (99 %), 3-methyl-1-butanol (≥98%), methylpropanal (≥98 %), 2methylthiophene (98 %), 2,3-pentanedione (≥96 %), phenylacetaldehyde (10 % in ethanol), 2phenylacetic acid (≥99 %), 2-phenylethanol (≥99 %), vanillin (≥97 %), 4-vinylphenol (10 % in propylene glycol). All were food grade except 2'-methoxyacetophenone and 2-methylthiophene.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!