Anti cd3e v450 500a2

Manufactured by BD

Anti–CD3e-V450 (500A2) is a laboratory product that can be used to detect and analyze CD3e-positive cells. It is a fluorochrome-conjugated antibody that binds to the CD3e antigen on the surface of T cells. The core function of this product is to facilitate the identification and characterization of T cell populations in samples through flow cytometry or other relevant analytical techniques.

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Lab products found in correlation

70 μm cell strainer, by Avantor (1 mentions) Anti cd19 pe cy7, by BD (1 mentions) Fcs express 6 flow cytometry software, by De Novo Software (1 mentions) Lsr flow cytometer, by BD (1 mentions) Rbc lysis buffer, by BioLegend (1 mentions) Stabilizing fixative, by BD (1 mentions) Armenian hamster igg, by Thermo Fisher Scientific (1 mentions) Mouse fc block, by BD (1 mentions)

2 protocols using anti cd3e v450 500a2

Immune Cell Isolation from Murine MLN

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Mechanical digestion of the MLN was achieved by a syringe plunger. After mechanical digestion, single cells were obtained by passing the cells through a 70-μm cell strainer (VWR International, Radnor, PA). Red blood cells (RBC) were lysed using RBC lysis buffer (BioLegend, San Diego, CA). Cells from 2 mice in the same experimental groups were combined to increase cell numbers for analyses. For T-cell populations, cells (1 × 10⁶ cells) were stained with antibodies anti–CD3e-V450 (500A2; BD Biosciences, Franklin Lakes, NJ) and anti–CD4-PE-Cy5 (RM4-5; BD Biosciences). For B-cell populations, cells (1 × 10⁶ cells) were labeled with anti–CD19-PE-Cy7 (1D3; BD Biosciences) and anti–MHC-II-FITC (2G9; BD Biosciences). Populations of T and B cells then were determined by a BD LSR flow cytometer (BD Biosciences ). Flow cytometry data were analyzed using FCS Express 6 Flow Cytometry Software (De Novo Software, Glendale, CA). The gating strategy used to determine immune cell populations is presented in Supplemental Figure 1.

Lin C.Y., Lee A.H., Chiu K.K., Vieson M.D., Steelman A.J, & Swanson K.S. (2020). Saccharomyces cerevisiae Fermentation Product Did Not Attenuate Clinical Signs, but Psyllium Husk Has Protective Effects in a Murine Dextran Sulfate Sodium–Induced Colitis Model. Current Developments in Nutrition, 4(11), nzaa159.

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Multicolor Flow Cytometry Immunophenotyping

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Cell suspensions in PBS + 0.5% BSA were pre-incubated with Mouse Fc Block (BD Biosciences) for 5 min at 4°C (0.5 μg/10⁶ cells/100 μl), then incubated with fluorophore-conjugated anti-mouse monoclonal antibodies for 30 min at 4°C (dilutions determined by titration). The cells were fixed in BD stabilizing fixative (BD Biosciences). Monoclonal antibodies specific for mouse hematopoietic surface lineage markers, or isotype-matched control antibodies, were used with the fluorophores V450 (eFluor450), PE, PE-Cy7, PerCP, FITC, AF647, and APC-eFluor 780 for seven color-labelling of cells. Anti-CD45-PerCP (clone 30-F11), anti-Ly6G-FITC (1A8), anti-Ly6C-PE or –PECy7 (AL-21), anti-CD11b-PECy7 (M1/70), anti-Siglec-F-PE (E50-2440), and anti-CD3e-V450 (500A2) were from BD Biosciences. Anti-CD11b-APCeFluor780 (M1/70), anti-F4/80-APCeFluor780 (BM8), anti-MHCII-APCeFluor780 (M5/114.15.2), anti-CD11c-AF647 or -eFluor450 (N418), anti-CD4-PECy7 or -AF647 (GK1.5) and anti-CD8a-PE (53-6.7) were from eBioscience. Appropriate conjugates of rat IgG2b, rat IgG2a, rat IgM, and Armenian hamster IgG (eBio299Arm) were used as isotype controls (eBioscience). Labeled cells were analyzed at the Flow Cytometry Core Facility, Massachusetts General Hospital, using a BD SORP 7 Laser LSRII and FlowJo software [30 ].

Winslow V., Vaivoda R., Vasilyev A., Dombkowski D., Douaidy K., Stark C., Drake J., Guilliams E., Choudhary D., Preffer F., Stoilov I, & Christmas P. (2014). Altered Leukotriene B4 metabolism in CYP4F18-deficient mice does not impact inflammation following renal ischemia. Biochimica et biophysica acta, 1841(6), 868-879.

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