TNF-α mouse uncoated enzyme-linked immunosorbent assay (ELISA) kit, IL-1β mouse uncoated ELISA kit, IL-10 mouse uncoated ELISA kit, IL-12 p70 mouse uncoated ELISA kit, interferon (IFN)-γ mouse uncoated ELISA kit, and IL-4 mouse uncoated ELISA kit were purchased from Invitrogen (ThermoFisher Scientific, USA). DCs (5 × 105 cells/mL) were incubated with 5, 10, or 15 μg/mL SARS-CoV-2 VLPs for 24 h; 50 ng/mL lipopolysaccharide-treated DCs were used as the positive control. The culture supernatants were collected and evaluated for the levels of TNF-α, IL-1β, IL-10, IL-12p70, IFN-γ, and IL-4 using the respective ELISA kit according to the manufacturer’s instructions. The levels of cytokines released into culture medium were determined by measuring OD450 using a microplate reader.
Il 12 p70 mouse uncoated elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The IL-12 p70 mouse uncoated ELISA kit is a laboratory product designed to measure the concentration of IL-12 p70 in mouse samples. It is an enzyme-linked immunosorbent assay (ELISA) kit that provides a quantitative in vitro assay for this analyte.
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2 protocols using il 12 p70 mouse uncoated elisa kit
1
SARS-CoV-2 VLP-induced Cytokine Production
2
Quantifying Immune Markers in Tumor Tissue
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Serum was collected in EDTA containing tubes (Sarstedt) and IL-12 levels were determined using the IL-12 p70 Mouse Uncoated ELISA Kit (Invitrogen). Isolated tumors were snap-frozen on dry ice. Before thawing, a 5 mm metal bead and 1 ml of lysis buffer (20 mM Tris HCl (pH 7.5), 0.5% Tween 20, 150 mM NaCl, Sigma protease inhibitors 1:100) were added to the tubes. Tumors were lysed using a TissueLyser (Qiagen) for 5 min at 25 Hz. After centrifugation, protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). CCL5 concentration in the tumor lysates was analyzed by ELISA (Mouse RANTES Uncoated ELISA Kit Invitrogen) and normalized to the determined total protein concentrations.
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