T2 cell line and CHO-K1 cell line were purchased from the American Type Culture Collection (ATCC, San Francisco, USA). K562 cell line and SW480 cell line were obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). T2 cell line was grown in IMDM medium (Gibco, life technologies) supplemented with 20% FBS (Gibco, life technologies). K562 cell line and SW480 cell line was cultured in RPMI-1640 medium (Gibco, life technologies) supplemented with 10% FBS. Cell lines were maintained in a humid atmosphere at 37 °C and 5% CO2 with 100 U/ml penicillin, and 100 µg/ml streptomycin. Maleimidocaproyl-valine-citrulline-monomethyl auristatin E (Mal-Val-Cit-MMAE) were synthesized by Levena Biopharm (Nanjing, Jiangsu, China). All reagents were purchased from Sigma-Aldrich (Saint Louis, MO, USA) unless otherwise noted.
Sw480 cell line
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SW480 cell line is a colorectal adenocarcinoma cell line derived from a primary tumor. It is commonly used in cancer research as a model system.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Cho k1 cell line, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Imdm medium, by Thermo Fisher Scientific (1 mentions)
T2 cell line, by American Type Culture Collection (1 mentions)
Penicillin streptomycin solution, by Beyotime (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
2 protocols using sw480 cell line
1
Cell Culture and Reagent Preparation
2
Culturing Human Cell Lines
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The human colorectal cancer cell line SW480 and the human embryonic kidney 293T cell line were purchased from ATCC (MA, USA). The HEK293T cell line was cultured in DMEM (C11995500BT, Gibco, USA) supplemented with 10% FBS (10099141, Gibco, USA) and 1% penicillin‐streptomycin solution (C0222, Beyotime, China). The SW480 cell line was cultured in RPMI 1640 medium (C11875500, Gibco, USA) supplemented with 10% FBS and 1% penicillin‐streptomycin solution. All cell lines were cultured at 5% CO2 and 37°C.
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