1 × 104 cells were seeded into 96‐well culture plates and incubated for 12–24 h at 37°C with 5% CO2. We subsequently added CBDCA (0, 10, 25, 50, 100, 150, or 200 μM). After 72 h incubation, cell viability was assayed by the Premix WST‐1 Cell Proliferation Assay System (Takara Bio Inc.) according to the manufacturer's instructions. The absorbance (450 and 650 nm) was measured using SH‐9000 Lab microplate reader (Hitachi) after 30–60 min incubation.
Sh 9000 lab microplate reader
Manufactured by Hitachi
Sourced in Japan
The SH-9000 Lab microplate reader is a compact and efficient instrument designed for absorbance measurement in microplates. It is capable of performing various types of absorbance-based assays such as ELISA, enzymatic, and cell-based assays. The SH-9000 features a high-quality optical system and provides reliable and accurate results for a wide range of applications in research and diagnostic laboratories.
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Lab products found in correlation
2 protocols using sh 9000 lab microplate reader
1
CBDCA Cytotoxicity Assay in 96-well Plates
2
Cell Proliferation Assay via WST-1
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Cell proliferation was assayed by the Premix WST-1 Cell Proliferation Assay System (Takara, Shiga, Japan) according to the manufacturer's instructions. The absorbance was measured at 450 and 690 nm using an SH-9000 lab microplate reader (Hitachi, Tokyo, Japan). Briefly, cells were trypsinized 96 h after siRNA transfection and 2 × 10 3 cells were seeded into 96-well plates. The WST-1 assay was conducted 96 h after seeding.
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