The samples of Poria were selected from seven provinces of China (Yunnan, Sichuan, Hubei, Hunan, Henan, Zhejiang, and Jiangxi). The sample authentication was performed by Professor Changqin Li at Henan University. A total of 12 batches of Poria samples were collected from different origins in 2019 without obvious different sensory characteristics, and the origin information is shown in Table 1 .
4-Nitrophenyl-α-D-glucopyranoside (Lot: 2875129) and α-glucosidase (Lot: G5003, activity: 1 KU) were purchased from Sigma-Aldrich (Darmstadt, Germany).
An LC-20AT HPLC system (Shimadzu, Kyoto, Japan) equipped with an LC solution chromatography workstation and a Thermo BDS HYPERSIL C18 column (4.6 mm × 250 mm, 5 μm) was used for the chromatographic analysis of Poria composition. A microplate reader (Multiskan MK3) was purchased from Thermo Electron (New York, USA). A UPLC-MS/MS system equipped with a Thermo Ultimate 3000 UHPLC system and a quadrupole-exactive-orbitrap mass spectrometer was purchased from Thermo Fisher Scientific (Waltham, USA).
4-Nitrophenyl-α-D-glucopyranoside (Lot: 2875129) and α-glucosidase (Lot: G5003, activity: 1 KU) were purchased from Sigma-Aldrich (Darmstadt, Germany).
An LC-20AT HPLC system (Shimadzu, Kyoto, Japan) equipped with an LC solution chromatography workstation and a Thermo BDS HYPERSIL C18 column (4.6 mm × 250 mm, 5 μm) was used for the chromatographic analysis of Poria composition. A microplate reader (Multiskan MK3) was purchased from Thermo Electron (New York, USA). A UPLC-MS/MS system equipped with a Thermo Ultimate 3000 UHPLC system and a quadrupole-exactive-orbitrap mass spectrometer was purchased from Thermo Fisher Scientific (Waltham, USA).