The NR8383 cells and BMDMs were plated in a 6-well plate. When cells reached 50% confluence, they were transfected with 10 nM NC-mimic, miR-183-5p mimic, NC-inhibitor, miR-183-5p inhibitor, siFoxO1 or si-NC (RiboBio, Guangzhou, China). Lipofectamine 3000 (11668019, Thermo) was used for transfection experiments according to the product instructions.
Mir 183 5p inhibitor
Manufactured by RiboBio
Sourced in China, United States
The MiR-183-5p inhibitor is a laboratory product designed to inhibit the expression of the microRNA miR-183-5p. This inhibitor can be used as a tool for research purposes to study the biological functions and regulatory mechanisms of miR-183-5p in various cellular and molecular processes.
Automatically generated - may contain errors
Lab products found in correlation
Inhibitor nc, by RiboBio (2 mentions)
Mir 183 5p mimic, by RiboBio (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Si foxo1, by RiboBio (1 mentions)
Si nc, by RiboBio (1 mentions)
Mimic nc, by RiboBio (1 mentions)
Mimics nc, by RiboBio (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
2 protocols using mir 183 5p inhibitor
1
Transfecting NR8383 cells and BMDMs
2
Cell Transfection with mepivacaine and miR-183-5p
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Cell transfection was performed as described previously [10 (link),11 (link)]. We seeded the SH-SY5Y cells treated with 10 mmol/L mepivacaine in a 6-well plate at a density of 5 × 105/cm2 and placed them at 37°C, 5% CO2 incubator. 16 h later, cell transfection was performed. MiR-183-5p inhibitor, inhibitor-NC, miR-183-5p mimics, and mimics-NC were procured from Guangzhou RiboBio Co, Ltd. We used Lipofectamine ® 2000 (Invitrogen, Carlsbad, CA, USA) for transfecting SH-SY5Y cells according to the supplier’s instructions. RT-qPCR was exploited for assaying transfection efficiency.
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