Bioluminizer

Manufactured by CAMAG

The BioLuminizer is a laboratory instrument that uses bioluminescence to detect and analyze biological samples. It measures the light output of luciferase-based reporter systems, providing quantitative data on gene expression and other biological processes.

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Lab products found in correlation

Tlc immersion device, by CAMAG (2 mentions) Lichrosolv, by Merck Group (1 mentions) Chromatogram immersion device 3, by CAMAG (1 mentions) Silica gel 60 f254 glass plates, by Merck Group (1 mentions) Tlc plate heater, by CAMAG (1 mentions)

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BioLuminizer 2 (2 citations)

4 protocols using bioluminizer

Bioluminescence Imaging of Bacterial Suspension

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According to Krüger
et al.,^{38 (link)} the dried chromatogram was immersed
into the bacterial suspension (prepared according to DIN EN ISO 11348-1,
part 1, section 5) for 2 s at an immersion speed of 2 cm s^–1 and placed into the Bioluminizer (CAMAG). Eleven luminescent plate
images were captured with a cooled 16 bit high-resolution CCD camera
(exposure time 50 s) in time intervals of 3 min until 30 min. To keep
the plate moist during the image recording, the HPTLC plate compartment
was humidified by a water-wetted filter paper (sized 15 × 10
cm² to cover the free space) and covered by a glass plate.

Stiefel C., Schubert T, & Morlock G.E. (2017). Bioprofiling of Cosmetics with Focus on Streamlined Coumarin Analysis. ACS Omega, 2(8), 5242-5250.

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Bioluminescence Assay for Antibacterials

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The chromatogram was immersed (immersion speed 3.5 cm/s and immersion time 2 s, TLC Immersion Device, CAMAG) in an Aliivibrio fischeri culture. The readiness of the bacterial growth prepared according to [43 ,44 (link)] was visually controlled for an emitted brilliant green-blue light by shaking the culture flask in a dark room. The bioluminescence was recorded with an exposure time of 50 s (time interval 3 min, BioLuminizer, CAMAG). Dark zones indicated antibacterial compounds.

Morlock G.E., Belay A., Heil J., Mehl A, & Borck H. (2022). Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry. Molecules, 27(11), 3541.

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HPTLC-Luminescent Bacteria Assay for Toxicity

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For the performance of the HPTLC coupled luminescent bacteria assay, liquid-dried bacteria were reactivated with 4 ml of supplied reactivation solution and cultivated in 200 ml liquid medium for pre- and main cultures according to DIN EN ISO 11348-1 (ISO 2007b ) in an Erlenmeyer flask with cap under constant agitation (350 ± 50 rpm) for 48 ± 2 h at room temperature. On the day of analysis, ethanolic extracts were sprayed in 5 mm bands using the automatic TLC sampler ATS 4 (Camag) on a HPTLC plate (Silica gel 60 F₂₅₄ glass plates, 20×10 cm, Merck Chemicals, prewashed with Methanol and activated by drying for 30 min at 110°C). Chromatographic development was conducted in the automated developing chamber AMD 2 (Camag) with ethyl acetate (Optigrade, Promochem) and n-hexane (Lichrosolv, Merck) (35:65, v/v) after focusing the samples with methanol. Before exposition with luminescent bacteria, the solvents on the plates were allowed to evaporate for at least 3 h. Subsequently, the plates were dipped into a suspension of luminescent bacteria with the Chromatogram Immersion Device 3 (Camag) for 1 s at highest speed. The supernatant suspension was removed from the silica surface using a squeegee. The bioluminescence was documented after an exposure time of 11 min by using a cooled 16 bit CCD camera integrated in the BioLuminizer (Camag). Toxic fractions appear as dark zones.

Bell A.M., Keltsch N., Schweyen P., Reifferscheid G., Ternes T, & Buchinger S. (2021). UV aged epoxy coatings – Ecotoxicological effects and released compounds. Water Research X, 12, 100105.

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Bioluminescent Bacterial Metabolism Monitoring

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The bioluminescent Aliivibrio fischeri suspension (evaluated upon shaking in a dark room) was sprayed on the HPTLC chromatogram, and the humid plate was transferred to the BioLuminizer (CAMAG) as described by Jamshidi-Aidji and Morlock [69 (link)]. Ten images of the bioluminescence were recorded over 30 min (exposure time 60 s, trigger interval 3.0 min), depicted as greyscale image. Dark zones revealed lower energetic metabolism of the bacteria, whereas bright zones indicated a higher energetic metabolism. As positive control, caffeine was used (1 mg/mL in methanol; 0.5, 1.5, and 3 μL/band).
The dried bioautogram was additionally derivatized by immersion in p-anisaldehyde sulfuric acid reagent (0.25 mL 4-methoxybenzaldehyde, 2 mL sulfuric acid, 4 mL glacial acetic acid, and 35 mL methanol) at 3 cm/s immersion speed for 2 s (TLC Immersion Device, CAMAG), followed by plate heating at 110 °C for 3 to 5 min (TLC Plate Heater, CAMAG) and detections under white light illumination and FLD 366 nm.

Oresanya I.O., Orhan I.E., Heil J, & Morlock G.E. (2024). African Under-Utilized Medicinal Leafy Vegetables Studied by Microtiter Plate Assays and High-Performance Thin-Layer Chromatography–Planar Assays. Molecules, 29(3), 733.

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