Blood agar with 7 sheep blood

Methanol was of analytical grade and supplied by Pronalab (Lisbon, Portugal) . n-Hexane (purity ≥ 99.0%) was purchased from Merck (Darmstadt, Germany). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Dulbecco's modified Eagle's minimum essential medium (DMEM), fetal bovine serum (FBS), Griess reagent system (Promega), DMSO, lipopolysaccharide (LPS), were obtained from Sigma-Aldrich Co. (Saint Louis, MO, USA). The bacterial strains (Eschericia coli ATCC 10536, Staphylococcus aures ATCC 19213 and Pseudomonas aeruginosa ATCC 9027) were purchased from Liofilchem Bacteriology Products (Italy). The culture media Muller Hinton broth (MHB) and Tryptic Soy Broth (TSB) were obtained from Biomerieux (Marcy l'Etoile, France). Blood agar with 7% sheep blood and MacConkey agar plates were purchased from Biomerieux Marcy l'Etoile, France). The dye p-iodonitrotetrazolium chloride (INT) was purchased from Sigma-Aldrich (Spruce Street; St. Louis, MO, USA) and was used as a microbial growth indicator. Helium, hydrogen, and synthetic air were acquired from Air Liquide (Portugal). The base cream was purchased from Fagron Iberica S.A.U. (Barcelona, Spain).

Sulforhodamine B, trypan blue, trichloroacetic acid (TCA), Tris, ellipticine, Streptomycin ampicillin, p-iodonitrotetrazolium chloride (INT) and the HPLC gradient grade solvents (acetonitrile, methanol and isopropanol) were purchased from Sigma (St. Louis, MO, USA). RPMI 1640 medium (HyClone), Hank's balanced salt solution (HBSS) and all the additional culture media components were purchased from Gibco Invitrogen Life Technologies (Paisley, UK). Gamma-oryzanol (CAS number 11042-64-1) was used as standard and purchased from TCI Europe. The culture media Muller Hinton broth (MHB) and Tryptic Soy Broth (TSB) were obtained from Biomerieux (Marcy l'Etoile, France). Blood agar with 7% sheep blood and Mac Conkey agar plates were purchased from bioMérieux (Marcy l'Etoile, France). Antifungal agents, bifonazole and ketoconazole, were purchased by Srbolek, Belgrade, Serbia and Zorkapharma, Sabac, Serbia, respectively. All other chemicals and solvents were of analytical grade and purchased from common sources. Water was treated in a Milli-Q water purification system (TGI PureWater Systems, Greenville, SC, USA).