Three jejunum cross-sections were prepared using standard paraffin embedding procedures by sectioning at a thickness of 5 μm and staining with hematoxylin and eosin. The jejunum villus height and crypt depth of the stained sections were examined with an Axioplan-2 optical microscope (Carl Zeiss Jena GmbH, Jena, Germany) coupled with a refrigerated QImaging Retiga 4000R digital camera (QImaging, Surrey, British Columbia, Canada) with a charge-coupled device detector, and were expressed in micrometers (μm). A total of 15 complete, well-oriented crypt-villus units were measured with an image processing and analysis system (Version 1, Leica Imaging Systems Co., Ltd., Cambridge, UK) for each type of tissue from each broiler.
Axioplan 2 optical microscope
Manufactured by Zeiss
Sourced in Germany
The Axioplan-2 is an optical microscope manufactured by Zeiss. It is designed for high-resolution imaging and analysis of various samples. The microscope features advanced optics and illumination systems to provide clear, detailed images. Its core function is to enable users to observe and study specimens at magnified levels.
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Lab products found in correlation
2 protocols using axioplan 2 optical microscope
1
Histological Analysis of Broiler Intestine
2
Microscopic Analysis of Formulations
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The procedure for microscopic analysis of the formulations was the same for all formulations. A small amount of formulations was placed on a glass slide, covered with a cover slip, and then subjected to microscopic analysis (Axioplan-2 Optical Microscope, Carl Zeiss ® ). The homogeneity of dispersion was evaluated and, with the aid of polarization, the presence of anisotropy areas, indicative of liquid crystals, was observed. The analyses were performed at 25 ºC.
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