Fitc conjugated goat anti rat igg

Manufactured by Jackson ImmunoResearch

Sourced in United States

FITC-conjugated goat anti-rat IgG is a secondary antibody that recognizes and binds to rat immunoglobulin G (IgG) molecules. The antibody is conjugated with the fluorescent dye fluorescein isothiocyanate (FITC), which allows for the detection and visualization of rat IgG in various immunological applications.

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Lab products found in correlation

Rat igg2a, by Thermo Fisher Scientific (1 mentions) Rat igg2b, by Thermo Fisher Scientific (1 mentions) Hamster igg, by BioLegend (1 mentions) Rat anti mouse f4 80 antibody, by Bio-Rad (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) Bz x700, by Keyence (1 mentions) Nis software, by Nikon (1 mentions) Tritc conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Eclipse ti, by Nikon (1 mentions)

3 protocols using fitc conjugated goat anti rat igg

Eosinophil Adhesion Molecule Expression

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Eosinophils were treated with t-TUCB (10 μM) or DMSO in PBS for 30 min at 37°C and then examined by flow cytometry for changes in expression of cell surface adhesion molecules using mAb against CD49d (α4, Clone PS/2), CD11a (αL, eBioscience, San Diego, CA), CD11b (αM, eBioscience), L-selectin (Clone MEL-14) or CD18 (β2, Clone 2E6) followed by FITC-conjugated goat anti-rat IgG (Jackson ImmunoResearch Laboratories, Inc.) or FITC-conjugated goat anti-hamster IgG (BioLegend, San Diego, CA, for CD18). Rat IgG2b (eBioscience, for CD49d and CD11b), rat IgG2a (eBioscience, for CD11a and L-selectin) or hamster IgG (BioLegend, for CD18) were used as isotype controls. All antibodies were used at 10 μg/ml.

Bastan I., Ge X.N., Dileepan M., Greenberg Y.G., Guedes A.G., Hwang S.H., Hammock B.D., Washabau R.J., Rao S.P, & Sriramarao P. (2018). Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation. Journal of leukocyte biology, 104(1), 109-122.

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Investigating Macrophage Response to miRNA in Colitis

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DSS-induced colitis was produced by free drinking of 2% DSS for 7 days in female mice (n = 2). Non-treated mice (n = 2) served as a comparative reference. The Alexia 647-tagged NC-miRNA (25 μg) encapsulated in sCA was injected into the tail vein and the distal colon was collected 4 h later, and frozen in OCT compound. About 8 μm sections were cut and fixed in 4% paraformaldehyde. The frozen sections (n = 6 per mouse) were incubated overnight with rat anti-mouse F4/80 antibody (BIO RAD, Cat, No. MCA497G, Hercules, CA, USA) at a concentration of 1:100. As a secondary antibody, FITC-conjugated goat anti-rat IgG was used (Jackson ImmunoResearch, Cat, No. 112-095-167, West Grove, PA, USA). The nuclei were stained with ProLong Gold anti-fade reagent with DAPI (Invitrogen, Cat, No. #8961). Sections were observed using a fluorescence microscope (BZ-X 700, Keyence Corporation, Osaka, Japan).

Tsujimura N., Ogino T., Hiraki M., Kai T., Yamamoto H., Hirose H., Yokoyama Y., Sekido Y., Hata T., Miyoshi N., Takahashi H., Uemura M., Mizushima T., Doki Y., Eguchi H, & Yamamoto H. (2023). Super Carbonate Apatite-miR-497a-5p Complex Is a Promising Therapeutic Option against Inflammatory Bowel Disease. Pharmaceuticals, 16(4), 618.

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Immunofluorescence Analysis of Meiotic Chromosomes

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Chromosome spreads for immunofluorescence analysis were prepared as described previously (56 ). The following primary antibodies were used in this study for immunostaining: monoclonal mouse SYCP3 antibody (diluted 1:500, Santa Cruz Biotechnology, SC-74569); polyclonal rat RPA antibody (diluted 1:200, Cell signaling, 2208). The following are the secondary antibodies were used in this study: FITC conjugated goat anti-rat IgG (diluted 1:500, Jackson Immuno Research, 112–095-003); TRITC conjugated goat anti-mouse IgG (diluted 1:500, Jackson Immuno Research, 115-025-003). Images were captured using a Super resolution microscope (Nikon Eclipse Ti) equipped with an EM CCD camera (iXon897, 100× Plan Apochromat lens, 1.49 NA). Images were deconvoluted and assembled into figures with the Nikon NIS software.

Joo J.H., Hong S., Higashide M.T., Choi E.H., Yoon S., Lee M.S., Kang H.A., Shinohara A., Kleckner N, & Kim K.P. (2024). RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination. Nucleic Acids Research, 52(7), 3794-3809.

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