Chemically defined lipid supplement

Manufactured by Thermo Fisher Scientific

Chemically defined lipid supplement is a specialized laboratory product that provides a defined mixture of lipids, intended to supplement cell culture media. It contains a specific combination of fatty acids, cholesterol, and other lipid components required for the growth and maintenance of cells in in vitro culture systems.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) B27 supplement, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Dehydroergosterol, by Merck Group (1 mentions) Taurine, by Thermo Fisher Scientific (1 mentions)

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Chemically Defined Lipid (10 citations) Defined lipids (3 citations) Lipid supplement (3 citations)

3 protocols using chemically defined lipid supplement

Fetal RPE Monolayer Cultivation

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Discarded de-identified human fetal eyes were collected for use in cell culture with permission from the UC Davis Institutional Review Board. Eyes were carefully dissected by removing the anterior segment, carefully removing the retina and peeling the RPE as a single sheet (monolayer) with the use of a dissecting microscope. Fetal RPEs were cultivated at 37°C under a humidified 5% CO₂ atmosphere in 3D-Retinal Differentiation Media (3:1 Dulbecco's modified Eagle medium (DMEM)-F12, DMEM (high glucose) supplemented with 5% (v/v) fetal bovine serum (Atlanta Biologicals), 2 mM GlutaMAX (Thermo Fisher Scientific), 200 μM taurine, 1× penicillin/streptomycin (Thermo Fisher Scientific), 1:1000 chemically defined lipid supplement (Thermo Fisher Scientific) and 2% B27 supplement (Thermo Fisher Scientific), as described previously (Capowski et al., 2019 (link)). All cultures were free of contamination at the time of analysis. No cell lines were used. Only primary tissues were expanded for the time indicated. Eyes were harvested from de-identified human fetal tissues without any known chromosomal abnormalities or birth defects.

Shao A., Lopez A.J., Chen J., Tham A., Javier S., Quiroz A., Frick S., Levine E.M., Lloyd K.C., Leonard B.C., Murphy C.J., Glaser T.M, & Moshiri A. (2022). Arap1 loss causes retinal pigment epithelium phagocytic dysfunction and subsequent photoreceptor death. Disease Models & Mechanisms, 15(7), dmm049343.

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Lipidomic Analysis of Cell Culture

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¹³C₁₈ Oleic acid, dehydroergosterol and C39:0 TAG were purchased from Sigma. C17:0 ceramide and d70 DSPC were purchased from Avanti lipids. LC-MS columns were obtained from Phenomenex. Chemically defined lipid supplement was purchased from ThermoFisher Sci (cat 11905031). The lipid supplement contains 8 different fatty acids with fatty acyl chain lengths ranging from C14-C20 (one of them being 2 mg/L; the rest of them are 10 mg/L) and cholesterol (220 mg/L) and was used at a final concentration of 1% in culture media. All other reagents were from Sigma-Aldrich.

Brovkovych V., Aldrich A., Li N., Atilla-Gokcumen G.E, & Frasor J. (2019). Removal of serum lipids and lipid-derived metabolites to investigate breast cancer cell biology. Proteomics, 19(18), e1800370.

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Fetal Retinal Pigment Epithelium Cultivation

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Discarded de-identified human fetal eyes were collected for use in cell culture with permission of the UC Davis IRB. Eyes were carefully dissected by removing the anterior segment, carefully removing the retina, and peeling the retinal pigmented epithelium as a single sheet (monolayer) with the use of a dissecting microscope. Fetal retinal pigment epithelia were cultivated at 37°C under a humidified 5% CO2 atmosphere in 3D-Retinal Differentiation Media (3:1 Dulbecco's modified Eagle medium (DMEM)-F12, DMEM (High Glucose) supplemented with 5% (v/v) Fetal Bovine Serum (Atlanta Biologicals), 2 mM GlutaMAX (Thermo Fisher), 200 μM Taurine, 1x Penicillin/Streptomycin (Thermo Fisher), 1:1000 chemically defined lipid supplement (Thermo Fisher), 2% B27 supplement (Thermo Fisher) as described previously. 12 (link)

Shao A., Lopez A.J., Chen J., Tham A., Javier S., Alonso A.Q., Frick S., Levine E.M., Lloyd K.C.K., Leonard B.C., Murphy C.J., Gläser T., & Moshiri A. (2021). Arap1 Loss Causes RPE Phagocytic Dysfunction and Subsequent Photoreceptor Death.

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