293 cells were lysed in RIPA (Solarbio, China) containing 1 mM PMSF (Solarbio). The protein concentration was evaluated by BCA Protein Assay Kit (Solarbio). Subsequently, 20 μg protein sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, USA). After blocking in 5% skimmed milk, the membranes were incubated with Rabbit anti-L.pneumophila polyclonal antibody (1:500, MyBioSource, USA) at 4 °C overnight. Then the membranes were incubated with Goat Anti-rabbit IgG/HRP antibody (1:3000, Solarbio) at 37 °C for 1 h. The bands were visualized by ECL solution (Solarbio).
Rabbit anti l pneumophila polyclonal antibody
Manufactured by MyBioSource
Sourced in United States
The Rabbit anti-L.pneumophila polyclonal antibody is a laboratory tool used for the detection and identification of the Legionella pneumophila bacteria. This antibody is produced by immunizing rabbits with L.pneumophila antigen, and the resulting polyclonal antibodies can be used in various immunoassay techniques to specifically recognize and bind to the target pathogen.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene difluoride membrane, by Merck Group (3 mentions)
Radioimmunoprecipitation assay (ripa), by Solarbio (3 mentions)
Ecl solution, by Solarbio (3 mentions)
Goat anti rabbit igg hrp antibody, by Solarbio (3 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (3 mentions)
Bca protein assay kit, by Solarbio (3 mentions)
3 protocols using rabbit anti l pneumophila polyclonal antibody
1
Western Blot Analysis of L. pneumophila
2
Immunoblotting Analysis of L. pneumophila
Check if the same lab product or an alternative is used in the 5 most similar protocols
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293 cells were lysed in RIPA (Solarbio, China) containing 1 mM PMSF (Solarbio). The protein concentration was evaluated by BCA Protein Assay Kit (Solarbio). Subsequently, 20 µg protein sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, USA). After being blocked with 5% skimmed milk, the membranes were incubated with Rabbit anti-L.pneumophila polyclonal antibody (1:500, MyBioSource, USA) at 4 °C overnight. Then the membranes were incubated with Goat Anti-rabbit IgG/HRP antibody (1:3000, Solarbio) at 37 °C for 1 h. The bands were visualized by ECL solution (Solarbio).
3
Western Blot Analysis of L.pneumophila
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
293 cells were lysed in RIPA (Solarbio, China) containing 1 mM PMSF (Solarbio). The protein concentration was evaluated by BCA Protein Assay Kit (Solarbio). Subsequently, 20 μg protein sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, USA).
After blocking in 5% skimmed milk, the membranes were incubated with Rabbit anti-L.pneumophila polyclonal antibody (1:500, MyBioSource, USA) at 4°C overnight. Then the membranes were incubated with Goat Anti-rabbit IgG/HRP antibody (1:3000, Solarbio) at 37°C for 1 h. The bands were visualized by ECL solution (Solarbio).
After blocking in 5% skimmed milk, the membranes were incubated with Rabbit anti-L.pneumophila polyclonal antibody (1:500, MyBioSource, USA) at 4°C overnight. Then the membranes were incubated with Goat Anti-rabbit IgG/HRP antibody (1:3000, Solarbio) at 37°C for 1 h. The bands were visualized by ECL solution (Solarbio).
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