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Kap1 s824

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KAP1-S824 is an antibody that specifically recognizes the phosphorylation of Serine 824 on the KAP1 (KRAB-associated protein 1) protein. KAP1 is a transcriptional regulator that plays a role in chromatin remodeling and gene expression. The phosphorylation of KAP1 at Serine 824 is involved in the cellular response to DNA damage.

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Lab products found in correlation

β actin, by Santa Cruz Biotechnology (2 mentions) Chk1 s317, by Cell Signaling Technology (2 mentions) P erk, by Cell Signaling Technology (1 mentions) H3k36me3, by Cell Signaling Technology (1 mentions) β tubulin, by Abcam (1 mentions) H3k4me2, by Cell Signaling Technology (1 mentions) Atm s1981, by Abcam (1 mentions) H3k9me2, by Cell Signaling Technology (1 mentions) H3k9ac, by Cell Signaling Technology (1 mentions) Hif 1α, by BD (1 mentions) Chk1 s345, by Cell Signaling Technology (1 mentions) Hif 2α, by Novus Biologicals (1 mentions)

Spelling variants of this product

), KAP1-S824 (#4127) and PPM1D (clone D4F7, (2 citations)

2 protocols using kap1 s824

1

DNA Damage Response Protein Analysis

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Cells were harvested in UTB lysis buffer (9 M Urea, 75 mM Tris-HCl pH 7.5, 0.15 M β-mercaptoethanol). Blots were visualised using the Odyssey Infrared imaging system. Antibodies used were SETX, KAP1 (Bethyl); GRP78, HIF1α, (ΒD Biosciences); ATM-S1981, RPA-S4/8, β-Tubulin (Abcam); ATM, RPA, KAP1-S824, p53-S15, H3K9me2, H3K4me2, H3K9Ac, H3-S10, H3, H3K36me3, Chk1-S317, PERK (Cell Signaling); p53, β-actin, RNase H1, Chk1 (Santa Cruz); and H3K9me3, γH2AX, H2AX (Millipore).
Ramachandran S., Ma T.S., Griffin J., Ng N., Foskolou I.P., Hwang M.S., Victori P., Cheng W.C., Buffa F.M., Leszczynska K.B., El-Khamisy S.F., Gromak N, & Hammond E.M. (2021). Hypoxia-induced SETX links replication stress with the unfolded protein response. Nature Communications, 12, 3686.
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2

Antibody Panel for DNA Damage Response

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The antibodies used were ATR-T1989 (Millipore), Chk1-S317, Chk1-S345, KAP1-S824 (Cell Signaling Technology), KAP1-S473 (Biolegend), KAP1 (Bethyl/Universal Biologicals Cambridge), Chk1, ATR, β -actin (Santa Cruz Biotechnology), HIF-2α (Novus Biologicals), HIF-1α(BD Biosciences) and GAPDH (Stratech Scientific).
Leszczynska K.B., Dobrynin G., Leslie R.E., Ient J., Boumelha A.J., Senra J.M., Hawkins M.A., Maughan T., Mukherjee S, & Hammond E.M. (2016). Preclinical testing of an Atr inhibitor demonstrates improved response to standard therapies for esophageal cancer. Radiotherapy and Oncology, 121(2), 232-238.
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