Rabbit anti suz12

Manufactured by Cell Signaling Technology

Rabbit anti-Suz12 is a primary antibody product used for the detection and analysis of the Suz12 protein in biological samples. Suz12 is a core component of the Polycomb Repressive Complex 2 (PRC2), which plays a crucial role in epigenetic regulation and chromatin remodeling. This antibody can be utilized in various immunoassay techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the Suz12 protein.

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SUZ12 (51 citations) Anti-SUZ12 (22 citations)

4 protocols using rabbit anti suz12

Western Blot Antibody Validation

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The following antibodies were used for the western blots: mouse anti-Brg1 (1:1000; Santa Cruz Biotechnology sc-17796), rabbit anti-Suz12 (1:1000; Cell Signaling 3737), rabbit anti-actin (1:500; Sigma A2066), rabbit anti-H3 (1:2000; Abcam), rabbit anti-BAF53a (1:1000; Novus NB100-61628 ), rabbit anti-Epc-1 (1:1000; Abcam ab5514), rabbit anti-Tip60 (1:500; Millipore 07-038), and mouse anti-β-actin (1:1000; Abcam ab6276). Antibodies against BAF subunits were generated in our laboratory and used at the following concentrations: BAF53b (1:500), BAF45d (1:2000), and BAF155 (1:1000). The secondary antibodies were goat anti-rabbit or mouse conjugated with IRDye 680RD or 800CW. Images were taken and quantified using an Odyssey/LiCor fluorescence imaging system.

Braun S.M., Petrova R., Tang J., Krokhotin A., Miller E.L., Tang Y., Panagiotakos G, & Crabtree G.R. (2021). BAF subunit switching regulates chromatin accessibility to control cell cycle exit in the developing mammalian cortex. Genes & Development, 35(5-6), 335-353.

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Western Blotting of OPCs and Brain Tissues

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For Western blotting, acutely isolated mouse OPCs or brain tissues were lysed in radioimmunoprecipitation assay buffer, containing protease and phosphatase inhibitors. Western blot analysis was performed as described previously. Mouse anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Millipore MAB374) was used as an input control. The antibodies used were mouse anti-EZH1 (Sigma-Aldrich, ABE281), rabbit anti-SUZ12 (Cell Signaling Technology, 13701S), rabbit anti-H3k27ac (Active Motif, 39135), rabbit anti-H3k27me3 (Cell Signaling Technology, 9733), rabbit anti-EZH2 (Cell Signaling Technology, 5246), rabbit anti-EED (EMD Millipore, 17-0034), goat anti-MBP (Santa Cruz Biotechnology, sc-13914), mouse anti-GFAP (Sigma-Aldrich, G3893), mouse anti-p53 (Santa Cruz Biotechnology, sc-126), and rabbit anti-p16^Ink4a (Santa Cruz Biotechnology, sc-467). Secondary antibodies conjugated to horseradish peroxidase were from Jackson ImmunoResearch Laboratories.

Wang J., Yang L., Dong C., Wang J., Xu L., Qiu Y., Weng Q., Zhao C., Xin M, & Lu Q.R. (2020). EED-mediated histone methylation is critical for CNS myelination and remyelination by inhibiting WNT, BMP, and senescence pathways. Science Advances, 6(33), eaaz6477.

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Western Blot and ChIP Antibodies

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The following primary antibodies were used in western blot, immunofluorescence or chromatin immunoprecipitation assays as indicated: mouse anti-c-Myc (ThermoFisher cat#9E10), mouse anti-His (Santa Cruz cat#sc57598), rabbit anti-EZH2 (Invitrogen cat#36–6300), rabbit anti-SUZ12 (Cell signaling cat#D39F6), rabbit anti-H3k27me3 (Active motif Cat#39155), rabbit anti-H3k4me3 (EMD Millipore cat#07–473), rabbit anti-βactin (Abcam cat#ab8227), rabbit anti-Histone H3 (Abcam cat#ab1791), mouse anti-Tax (cat#168-A51 from the National Institutes of Health AIDS Research and Reference Reagent Program), mouse anti-HBZ (a gift from J.M. Péloponèse), rabbit anti-E(z), rabbit anti-SUZ12 (a gift from J. Müller), mouse anti-Relish (DSHB cat#C21F3) and anti-GAPDH (B2534M-HRP Abnova), mouse anti-c-Myc-tag (9E10) (Abcam cat#ab32), rabbit anti-EZH2 (Merck Millipore cat# 07–689), Goat anti-mouse Alexa Fluor-488 (Abcam cat≠150113), Goat anti rabbit -rabbit Alexa Fluor-594 (Abcam cat ≠150080).

Akkouche A., Moodad S., Hleihel R., Skayneh H., Chambeyron S., El Hajj H, & Bazarbachi A. (2021). In vivo antagonistic role of the Human T-Cell Leukemia Virus Type 1 regulatory proteins Tax and HBZ. PLoS Pathogens, 17(1), e1009219.

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Western Blot Antibody Analysis

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The following antibodies were used for the western blots: mouse anti-Brg1 (Santa Cruz, sc-17796, 1:1000), rabbit anti-Suz12 (Cell Signaling, 3737, 1:1,000), rabbit anti-actin (Sigma, A2066, 1:500), rabbit anti-H3 (Abcam, 1:2,000), rabbit anti-BAF53a (Novus, NB100-61628, 1:1,000), rabbit anti-Epc-1 (Abcam, ab5514, 1:1000), rabbit anti-Tip60 (Millipore, 07-038, 1:500), mouse anti-β-actin (Abcam, ab6276, 1:1000). Antibodies against BAF subunits were generated in our laboratory and used at the following concentrations: BAF53b (1:500), BAF45d (1:2,000) and BAF155 (1:1,000). The secondary antibodies were goat anti-rabbit or mouse conjugated with IRDye 680RD or 800CW. Images were taken and quantified by Odyssey Quantitative Fluorescence Imaging Systems.

Braun S.M.G., Petrova R., Tang J., Krokhotin A., Miller E.L., Tang Y., Panagiotakos G., & Crabtree R.H. (2020). The npBAF to nBAF Chromatin Switch Regulates Cell Cycle Exit in the Developing Mammalian Cortex.

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