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3 protocols using ab30931

1

Comprehensive Antibody Validation Protocol

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Antibodies against Necdin (ab18554), SGT1 (ab30931), BMAL1 (ab3350), HSP90 (ab13492), PER1 (ab136451), PER2 (ab180655), CRY1 (ab104736) were purchased from Abcam. Antibody against AVP (sc-390723) were obtained from Santa Cruz. Antibodies against Myc-tag (2276), Flag-tag (14793), HA-tag (3724), VIP (63269) were obtained from Cell Signaling Technology. Antibody against CRY2 were obtained from Invitrogen. Antibody against GRP (20073) were obtained from ImmunoStar. 17-AAG (HY-10211) and Geldanamycin (HY-15230) were purchased from MedChemExpress.
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2

Antibody Panel for Protein Analysis

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Anti-PHLPP1 (A300-660A from Bethyl Laboratories and 07-1341 from Millipore), anti-SGT1 (A302-944A from Bethyl Laboratories and Ab30931 from Abcam), anti-RNF41 (A300-048A from Bethyl Laboratories), anti-CENP-A (Ab13939 from Abcam), anti-CENP-E (Ab5093 from Abcam), anti-HEC1 (Ab3613 from Abcam) anti-Myc, clone 9E10 (SC-40 from Santa Cruz Biotechnology), anti-histone H3 (05928 from Millipore), anti-γ-tubulin (T5326 from Sigma), anti-α-tubulin (T6074 from Sigma), anti-FLAG (F3165 from Sigma), anti-actin (A5441 from Sigma), anti-HA (A190-108A from Bethyl Laboratories), and anti-ubiquitin (05-944 from Millipore) antibodies were used in this study.
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3

Antibody Immunostaining for SEMA3A, PAK, and pFAK

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Antibodies against SEMA3A (ab18554), PAK (ab30931), and pFAK (ab3350) were purchased from Abcam.
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