Astaxanthin and dimethyl sulfoxide were purchased from Sigma-Aldrich Inc. (Taufkichen, Germany). Cell Counting Kit-8 (CCK-8) was purchased from DOJINDO Inc. (Kumamoto, Japan). ROS Detection Assay Kit was purchased from Biovision Inc. (Milpitas, CA, USA). PE Annexin V apoptosis detection kit was purchased from BD Biosciences Inc. (San Jose, CA, USA). Anti-BAX pAb, anti-BCL2 mAb, anti-cleaved CASP3 mAb, and anti-cleaved PARP mAb were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Anti-ACTB pAb were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).
Anti bcl2 mab
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-BCL2 mAb is an antibody that binds to the BCL2 protein. BCL2 is a regulator of apoptosis, or programmed cell death. This antibody can be used to detect and analyze the BCL2 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Astaxanthin, by Merck Group (1 mentions)
Anti cleaved parp mab, by Cell Signaling Technology (1 mentions)
Ros detection assay kit, by Abcam (1 mentions)
Anti bax pab, by Cell Signaling Technology (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Pe annexin 5 apoptosis detection kit, by BD (1 mentions)
Anti bax mab, by Cell Signaling Technology (1 mentions)
Goat anti mouse mfg e8 antibody, by R&D Systems (1 mentions)
Hrp conjugated secondary antibody, by Southern Biotech (1 mentions)
Anti β actin mab, by Cell Signaling Technology (1 mentions)
2 protocols using anti bcl2 mab
1
Astaxanthin-Induced Apoptosis and ROS Modulation
2
Western Blot Analysis of Apoptotic Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins from the lung tissues were separated by SDS-PAGE, and the MFG-E8 protein was detected with a goat anti-mouse MFG-E8 antibody (R&D Systems). The lung proteins were incubated with rabbit anti-Bcl-2 mAb, anti-Bcl-XL mAb, anti-Bax mAb and anti-β-actin mAb (Cell Signaling Technology, Danvers, MA, USA) and then visualized with an HRP-conjugated secondary antibody (Southern Biotech, UAB, Birmingham, AL, USA) and chemilumiescence (Thermo Pierce, Rockford, IL, USA) to further detect apoptosis.
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