For Western blot, the primary antibodies were as follows: rabbit anti AXL (1:500, bs-5180R, Bioss, Beijing, China), rabbit anti GAS6 (1:500, bs-7549R, Bioss), mouse anti GFAP (1:1000, MAB360, Millipore), rabbit anti CD68 (1:500, bs-0649R, Bioss), mouse anti ABCA1 (1:500, ab18180, Abcam, Cambridge, United Kingdom), rabbit anti IL-1β (1:500, ab9722, Abcam), rabbit anti TNFα (1:500, ab6671, Abcam), rabbit anti IL-6 (1:500, ab7737, Abcam), rabbit anti STAT1 (1:1000, 14994, CST), rabbit anti phospho-STAT1 (Tyr701) (1:1000, 9167, CST), and β-actin (1:1000, ab8227, Abcam). For immunofluorescence, the following antibodies were used: mouse anti GFAP (1:500, MAB360, Millipore), mouse anti NeuN (1:500, ab104224, Abcam), goat anti Iba-1 (1:500, ab5076, Abcam), rabbit anti AXL (1:500, bs-5180R, Bioss), rabbit anti GAS6 (1:500, bs-7549R, Bioss), and mouse anti ABCA1 (1:500, ab18180, Abcam). For flow cytometry, ACSA-2 antibody, anti-mouse, APC (1:50, 130-117-53, Miltenyi Biotec) and isotype control antibody, rat IgG2b, APC (1:50, 130-123-825, Miltenyi Biotec).
Zhou H., Hu L., Li J., Ruan W., Cao Y., Zhuang J., Xu H., Peng Y., Zhang Z., Xu C., Yu Q., Li Y., Dou Z., Hu J., Wu X., Yu X., Gu C., Cao S., Yan F, & Chen G. (2021). AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury. Journal of Neuroinflammation, 18, 154.
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