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Sc 72578

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Sc-72578 is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is designed for general laboratory use. The core function of this product is to facilitate common laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Dharmafect 1 transfection reagent, by Thermo Fisher Scientific (1 mentions) Sc 41445, by Santa Cruz Biotechnology (1 mentions) Sc 7020, by Santa Cruz Biotechnology (1 mentions) Sc 44230, by Santa Cruz Biotechnology (1 mentions) Mk 2206 2hcl s1078, by Selleck Chemicals (1 mentions) Sc 29679, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sc 72578

1

Knockdown of Autophagy Genes in Caco-2 Cells

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Small interfering RNAs (siRNAs) specific for ATG12 (human, sc-72578), ATG5 (human, sc-41445), along with a control siRNA (sc-44230), each with 19–25 nucleotides, were obtained from Santa Cruz Biotechnology (Texas, USA). Caco-2 cells were transfected with 100 nM siRNA (ATG12 and ATG5) and 10 nM Dharmafect 1 transfection reagent (Thermo Scientific, T-2001-03) for 24 hours.
Tang B., Wang K., Jia Y.P., Zhu P., Fang Y., Zhang Z.J., Mao X.H., Li Q, & Zeng D.Z. (2016). Fusobacterium nucleatum-Induced Impairment of Autophagic Flux Enhances the Expression of Proinflammatory Cytokines via ROS in Caco-2 Cells. PLoS ONE, 11(11), e0165701.
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2

Autophagy Regulation in Host-Pathogen Interactions

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Rapamycin (Rapa, R8781), 3-methyladenine (3-MA, M9281), bafilomycin A1 (Baf-A1, B1793), antibodies against ATG12 (WH0009140M1), MAP1LC3B (L7543), and ATG5 (WH0009474M1) were purchased from Sigma-Aldrich (Shanghai, CHINA), and MK-2206 2HCL (S1078) purchased from Selleckchem (Houston, TX, USA). Antibodies against Akt (9272), mTOR (2972), AMPK (2532), phospho-Akt (Ser473) (4060), LAMP1 (9091), phospho-mTOR (Ser2448) (5536), phospho-S6 ribosomal protein (Ser235/236) (2211), ribosomal protein (2217), phospho-c-Met (Y1234/Y1235) (4033), and c-Met (4560) were obtained from Cell Signaling Technology (Beverly, MA, USA), whereas antibodies against β-actin (sc-10731), VacA (sc-25790), CagA (sc-17450), phospho-tyrosine (PY99) (sc-7020) and SQSTM1 (sc-28359), and siRNAs specific for SQSTM1 (human, sc-29679), ATG12 (human, sc-72578), c-Met (human, sc-29397), and ATG5 (human, sc-41445), along with a control siRNA (sc-44230) were obtained from Santa Cruz Biotechnology (CA, USA).
Li N., Tang B., Jia Y.P., Zhu P., Zhuang Y., Fang Y., Li Q., Wang K., Zhang W.J., Guo G., Wang T.J., Feng Y.J., Qiao B., Mao X.H, & Zou Q.M. (2017). Helicobacter pylori CagA Protein Negatively Regulates Autophagy and Promotes Inflammatory Response via c-Met-PI3K/Akt-mTOR Signaling Pathway. Frontiers in Cellular and Infection Microbiology, 7, 417.
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