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24 well transwell inserts 8 μm pore

Manufactured by Corning
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Sourced in United States

The 24-well transwell inserts (8 μm pore) are a laboratory equipment product designed for cell culture and research applications. These inserts feature a 24-well format and have a pore size of 8 μm, allowing for the study of cell migration, invasion, and permeability.

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Lab products found in correlation

Crystal violet, by Merck Group (1 mentions)

Close spelling variants of this product

Transwell (24-well insert; pore size 8 μm, Transwell chambers (24-well insert; 8-μm pore size; 24-well transwell 8-μm pores Transwell inserts Pore transwells 24 wells

2 protocols using 24 well transwell inserts 8 μm pore

1

Transwell Assay for Cell Migration

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Cell migration assay was performed using 24-well transwell inserts (8 μm pore; Corning, NY, USA) according to the manufacturer's instructions. RWPE-1 cells (105 cells/well) were seeded in the upper chamber of transwell plates and THP-1 macrophages (105 cells /well) or control medium was added to the lower chamber. Cells were incubated for 24 h with or without HX109 treatment. The cells migrated to the lower part of the membrane were stained with 0.2% crystal violet and counted in six random fields.
Lim S., Kim H.K., Lee W, & Kim S. (2020). Botanical preparation HX109 inhibits macrophage-mediated activation of prostate epithelial cells through the CCL4-STAT3 pathway: implication for the mechanism underlying HX109 suppression of prostate hyperplasia. Heliyon, 6(6), e04267.
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2

Transwell Assay for Cell Migration

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Transwell assay was performed using 24-well transwell inserts (8 μm pore, Corning, Unites States). 2 x 103 HFF were plated into the upper chamber, then cultured in 500 μL complete medium (containing 10% FBS) supplementing LF-FVs at the indicated concentrations (0, 10, 50, 100, 250 μg/mL). After incubation for 24 h, non-migrated cells were removed by cotton swab and migrated cells were fixed with 4% paraformaldehyde and stained with 0.1% crystal violet (Sigma, United States). The number of migrated cells was calculated under an optical microscope at a ×100 magnification (Olympus).
Wu Y., Hong P., Liu P., Zhang Q., Zhang Y., Yang B., Liu H., Liu L., Tian W, & Yu M. (2023). Lipoaspirate fluid derived factors and extracellular vesicles accelerate wound healing in a rat burn model. Frontiers in Bioengineering and Biotechnology, 11, 1185251.
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